Hydrolyzed Hemoglobin Supplement for Rumen Bacterial Mass
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Solution Overview
Problem
Current methods for maximizing bacterial growth in the rumen of ruminants, such as adding amino acids and peptides, do not effectively stimulate the growth of both Gram-positive and Gram-negative bacteria, and do not significantly increase microbial mass and milk production.
Innovation Solution
The method involves adding hydrolyzed proteins with a degree of hydrolysis above 28% and/or more than 23 mg α-amino nitrogen per gram of protein to the ruminant's diet, preferably animal proteins like hemoglobin, to provide a varied amino acid, peptide, and oligopeptide profile that stimulates bacterial growth.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If amino acids and peptides are added to the rumen diet, then bacterial growth is increased, but the stimulation is not sufficient for both Gram-positive and Gram-negative bacteria simultaneously
Solution Approach 1:
The patent changes the parameter of protein hydrolysis degree from conventional levels to above 28%, and specifies α-amino nitrogen content greater than 23 mg/g protein. This parameter change creates a hydrolyzed protein product with a unique amino acid profile that simultaneously stimulates both Gram-positive and Gram-negative bacteria, resolving the contradiction between bacterial mass increase and broad bacterial type coverage.
Solution Approach 2:
The patent uses composite material by combining hydrolyzed proteins with specific characteristics (high degree of hydrolysis >28%, high α-amino nitrogen >23 mg/g) with the ruminant diet. This composite approach provides a varied profile of amino acids, peptides, and oligopeptides that collectively stimulate diverse bacterial populations in the rumen, addressing the limitation of single-type supplements.
2Productivity
If conventional protein supplements are used, then some bacterial growth occurs, but microbial mass and milk production are not significantly increased
Solution Approach 1:
The patent applies parameter changes by specifying precise thresholds for hydrolysis degree (>28%) and α-amino nitrogen content (>23 mg/g protein). These parameter specifications create a highly effective supplement that significantly increases microbial mass, which in turn drives improved milk production, overcoming the limitations of conventional protein supplements.
3Quantity of substance
If highly hydrolyzed proteins are added, then bacterial growth is significantly stimulated, but the complexity of protein hydrolysis process increases
Solution Approach 1:
The patent resolves the complexity issue by defining clear parameter thresholds (degree of hydrolysis >28%, α-amino nitrogen >23 mg/g protein) that guide the hydrolysis process. These specified parameters provide a standardized target for protein hydrolysis, making the complex process controllable and reproducible while achieving significant bacterial growth stimulation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases the growth of both Gram-positive and Gram-negative bacteria, particularly Gram-negative bacteria, leading to improved microbial mass and milk production in ruminants, as demonstrated by in vitro studies and dairy cow experiments.
Implementation Method 1
A large proportion of the digestible diet of a ruminant is converted to the end products by way of microbial fermentation, basically volatile fatty acids (VFA), and the majority of the protein is converted to microbial protein.
Implementation Method 2
The majority of animal and vegetable proteins can be hydrolyzed either by enzymatic or acid methods.
Data Source
AI summary
A nutritional supplement, a method for increasing the bacterial mass in the rumen of a ruminant and a nutritional preparation and corresponding uses. A nutritional supplement comprising proteins hydrolyzed with a degree of hydrolysis of above 28% and/or which have more than 23 mg α-amino nitrogen per gram of protein and/or have more than 10% of free amino acids. The hydrolyzed proteins are animal proteins, preferably hemoglobin. The supplement is supplied orally, included in a nutritional preparation, in liquid, concentrated or dehydrated form. The supplement is the basis for a method of increasing the bacterial mass in the rumen of a ruminant in which there is added to the ruminant's diet a supplement with hydrolyzed proteins according to the invention.