Chromatography Separation Material with Hydrophilic Coating
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Solution Overview
Problem
Conventional separation materials fail to achieve both high protein adsorption and excellent column characteristics such as liquid permeability when used in chromatography.
Innovation Solution
A separation material comprising porous polymer particles with a coating layer of a macromolecule having hydroxyl groups, where the particles are synthesized using divinylbenzene as a monomer unit and a porosifier, and the coating layer is crosslinked to enhance durability and reduce non-specific protein adsorption.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If an ion exchanger having a porous type synthetic macromolecule as a matrix is used, then pressure resistance is improved, but non-specific adsorption occurs causing peak asymmetrization and protein loss
Solution Approach 1:
The invention uses a composite structure where a crosslinked gel of hydrophilic natural macromolecule (providing non-specific adsorption resistance) is filled within the pores of a porous synthetic macromolecule matrix (providing pressure resistance). This composite approach combines the advantages of both materials to resolve the contradiction between pressure resistance and non-specific adsorption.
2Object-generated harmful factors
If ion exchangers having crosslinked gels of hydrophilic natural macromolecule are used, then non-specific adsorption is reduced, but volume change and mechanical strength are worsened
Solution Approach 1:
The crosslinked gel of hydrophilic natural macromolecule is embedded within the rigid porous synthetic macromolecule matrix, which acts as a skeleton to constrain the gel's volume expansion. This composite structure allows the gel to maintain its low non-specific adsorption property while the synthetic matrix provides dimensional stability and reduces volume change.
3Object-generated harmful factors
If ion exchangers having crosslinked gels of hydrophilic natural macromolecule are used, then non-specific adsorption is reduced, but mechanical strength is worsened
Solution Approach 1:
The porous synthetic macromolecule matrix serves as a mechanically strong skeleton that provides structural support and mechanical strength to the overall ion exchanger structure, while the crosslinked gel of hydrophilic natural macromolecule filled in the pores provides the chemical property of reduced non-specific adsorption. Each component compensates for the other's weakness.
4Object-generated harmful factors
If crosslinked gels are used in chromatography, then non-specific adsorption is reduced, but pressure loss increases and gel consolidates
Solution Approach 1:
The rigid porous synthetic macromolecule matrix provides a stable framework with controlled pore size and distribution that maintains consistent liquid flow pathways, preventing gel consolidation and reducing pressure loss. The crosslinked gel filling the pores maintains its porous structure within this framework, enabling low non-specific adsorption without excessive pressure loss.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The material exhibits reduced non-specific adsorption, excellent alkali resistance, and improved column characteristics, including high protein adsorption capacity and liquid permeability, making it suitable for protein separation and purification.
Implementation Method 1
non-specific adsorption such as irreversible adsorption based on hydrophobic interaction occurs
Implementation Method 2
these ion exchangers have a defect that the ion exchangers swell conspicuously in aqueous solutions, undergo a large volume change due to the ionic strength of a solution
Implementation Method 3
when the ion exchanger is packed into a column and used for chromatography, the ion exchanger tends to have excellent pressure resistance at the time of liquid permeation
Data Source
Figure 1
AI summary
The present invention provides a separation material that comprises porous polymer particles comprising a styrene-based monomer as a monomer unit; and a coating layer comprising a macromolecule having hydroxyl groups, which covers at least a portion of the surface of the porous polymer particles, and the separation material has a 5% compressive deformation modulus of 100 to 1,000 MPa, and has a mode diameter in the pore size distribution of 0.1 to 0.5 µm.