Hydrophilic Polymer Particles for Low Non-Specific Adsorption
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Solution Overview
Problem
Existing polymer particles used in latex agglutination methods suffer from non-specific adsorption of contaminants, leading to decreased measurement accuracy and reproducibility, which is not adequately addressed by methods that use bovine serum albumin (BSA) to suppress such adsorption.
Innovation Solution
A method involving the production of polymer particles through a process that includes mixing a radically polymerizable monomer, an organic silane compound with an alkoxy group, a radical polymerization initiator, and a water-soluble polymer in an aqueous medium, followed by adding a reactive compound represented by a specific formula, which enhances hydrophilicity and suppresses non-specific adsorption without using BSA.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If BSA is used to cover the particle surface to suppress non-specific adsorption, then the adsorption of contaminants is reduced, but the reproducibility decreases and measurement accuracy is compromised
Solution Approach 1:
The invention extracts and eliminates BSA from the particle surface coating system. Instead of using BSA to cover the particle surface, the patent employs a different approach by incorporating hydrophilic groups directly into the polymer particle structure during synthesis, thereby removing the harmful element (BSA) that causes reproducibility issues while maintaining its protective function
Solution Approach 2:
The invention changes the chemical parameters of the particle surface by incorporating hydrophilic groups (carboxyl, hydroxyl, amino groups) directly into the polymer structure during emulsion polymerization. This parameter change transforms the surface properties from hydrophobic to hydrophilic, enabling suppression of non-specific adsorption without requiring BSA coating, thus improving measurement reproducibility
2Ease of operation
If polymer particles are used in latex agglutination methods, then the reaction can proceed, but non-specific adsorption of contaminants occurs leading to decreased measurement accuracy
Solution Approach 1:
The invention performs preliminary action by incorporating hydrophilic groups into the polymer particle structure during the synthesis stage, before the particles are used in latex agglutination reactions. This preliminary modification of the particle surface properties prevents non-specific adsorption of contaminants during subsequent measurements, thereby maintaining measurement accuracy while enabling the latex agglutination reaction to proceed
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The produced particles exhibit high hydrophilicity, improved uniformity, and enhanced reproducibility in suppressing non-specific adsorption, thereby maintaining measurement accuracy and stability in latex agglutination reactions.
Implementation Method 1
an organic silane compound having a silicon atom to which an alkoxy group is bonded
Implementation Method 2
an organic silane compound having a silicon atom to which an alkoxy group is bonded and having radical polymerizability
Implementation Method 3
a radically polymerizable monomer, an organic silane compound having a silicon atom to which an alkoxy group is bonded and having radical polymerizability, a radical polymerization initiator
Implementation Method 4
a water-soluble polymer, and an aqueous medium to prepare an emulsion
Data Source
AI summary
To provide a method for producing a particle that can suppress non-specific adsorption without using BSA. A method for producing a particle, including: a first step of mixing a radically polymerizable monomer, an organic silane compound having a silicon atom to which an alkoxy group is bonded and having radical polymerizability, a radical polymerization initiator, a water-soluble polymer, and an aqueous medium to prepare an emulsion; and a second step of adding a specific reactive compound after the first step.