Hydrophilic Silane Substrate for Cell Capture
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Solution Overview
Problem
Existing hydrophilic substrates with special polymers often have surface irregularities that hinder the efficient capture of specific cells, such as cancer cells, and are not suitable for use in aqueous solutions.
Innovation Solution
A hydrophilic substrate with a highly smooth hydrophilic silane compound layer formed on a hydrophilized surface, using alkaline solution treatment, ultraviolet irradiation, electron beam irradiation, or plasma treatments, and a specific silane compound formula (R21-O-(CpH2pO)m-(CH2)k-Si(X11)n(X12)3-n, which is applied within three hours of hydrophilization, providing a controlled surface for cell capture.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If special polymers are coated on substrate surface to capture specific cells, then cell capture function is provided, but surface irregularities occur which reduce capture ability
Solution Approach 1:
The invention changes the material parameter from special polymers to hydrophilic silane compounds, and controls the thickness parameter of the coating layer to 1-50 nm to achieve smooth surface while maintaining cell capture function
Solution Approach 2:
The invention uses composite structure combining hydrophilized surface treatment with hydrophilic silane compound coating to achieve both smoothness and cell capture ability
2Reliability
If substrates are provided with controlled surface irregularities for cell capture, then cell capture ability improves, but performance in aqueous solutions deteriorates
Solution Approach 1:
The invention changes the surface chemical parameter by applying hydrophilic treatment and silane coating, making the surface adaptable to aqueous environments while maintaining controlled irregularities for cell capture
Solution Approach 2:
The invention applies local hydrophilic modification to the substrate surface through silane coating, creating regions with specific properties that enable both cell capture and aqueous solution compatibility
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The smooth hydrophilic substrate effectively captures specific cells with reduced surface irregularities and improved adhesion, allowing for accurate cell counting and drug screening, while maintaining stability in solvents.
Implementation Method 1
a hydrophilic silane compound layer provided on a hydrophilized surface of a substrate
Implementation Method 2
the hydrophilization is preferably an alkaline solution treatment
Implementation Method 3
the hydrophilization is preferably at least one selected from the group consisting of ultraviolet irradiation, electron beam irradiation, ion irradiation, and plasma treatments
Data Source
AI summary
Provided are a hydrophilic substrate provided with a highly smooth hydrophilic silane compound layer having less surface irregularities and a method of manufacturing the hydrophilic substrate. The present invention relates to a hydrophilic substrate including a substrate having a hydrophilized surface and a hydrophilic silane compound layer provided on at least part of the hydrophilized surface of the substrate.