Hydrotropic Release Reagent for Vitamin D Quantification
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Solution Overview
Problem
Current methods for releasing vitamin D from its binding complex with vitamin D-binding protein (DBP) are labor-intensive, difficult to automate, and prone to faults, making direct quantitative determination challenging.
Innovation Solution
A reagent containing hydrotropic substances and transition metal salts is used to efficiently release vitamin D from protein complexes, allowing for direct immunological determination without additional sample treatment, enabling a reliable and automatable method for vitamin D quantification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods (organic solvents, phase-forming solvents, alkaline reagents) are used to release vitamin D from DBP, then vitamin D can be released from the binding complex, but the methods become labor-intensive, difficult to automate, and prone to faults
Solution Approach 1:
The patent changes the chemical parameters of the release reagent by combining hydrotropic substances with transition metal salts, creating a reagent system that releases vitamin D from DBP under mild, controlled conditions that are suitable for automation while maintaining high reliability
Solution Approach 2:
The transition metal salt acts as an intermediary that facilitates the release of vitamin D from the DBP complex through metal-vitamin D interactions, enabling reliable release without requiring harsh conditions that complicate automation
2Reliability
If conventional release methods are used, then vitamin D can be released from DBP, but additional sample treatment steps are required, increasing complexity and time
Solution Approach 1:
The patent merges the release function and determination function into a single integrated reagent system, allowing vitamin D to be released from DBP and subsequently determined in one procedure without requiring separate treatment steps, thereby reducing complexity while maintaining reliability
3Reliability
If conventional release methods are used, then vitamin D can be released from DBP, but the methods are time-consuming and not suitable for high-throughput analysis
Solution Approach 1:
The patent optimizes the chemical parameters of the release reagent to achieve rapid, efficient release of vitamin D from DBP under mild conditions, significantly reducing the time required for analysis while maintaining high reliability, thus enabling high-throughput productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves efficient release of vitamin D, reducing the complexity and time required for determination, providing a cost-effective and efficient means for quantifying 25-hydroxy vitamin D3 or 25-hydroxy vitamin D2 in biological samples.
Implementation Method 1
a release reagent which contains at least one hydrotropic substance and at least one transition metal salt
Implementation Method 2
at least one transition metal salt
Data Source
AI summary
The present invention relates to a method for releasing bound vitamin D by bringing a sample containing vitamin D into contact with a release reagent which contains at least one hydrotropic substance and at least one transition metal salt. The released vitamin D can subsequently be determined quantitatively. The present invention relates further to a reagent for releasing and optionally determining vitamin D, containing at least one hydrotropic substance and at least one transition metal salt, as well as to the use of such a release reagent for releasing and optionally determining vitamin D.