Enzymatic Production of Hydroxy Polyunsaturated Fatty Acid Derivatives
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing mono- or di-hydroxy derivatives of polyunsaturated fatty acids are complex and inefficient, limiting their availability for therapeutic use in alleviating skin wrinkles, inflammation, and strengthening skin barriers.
Innovation Solution
An enzyme with a specific amino acid sequence is used to produce mono- or di-hydroxy derivatives of polyunsaturated fatty acids, which are then incorporated into cosmetic, food, or pharmaceutical compositions to improve skin conditions, including wrinkle prevention, inflammation reduction, and skin barrier strengthening.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If organic synthesis process is used to produce SPM, then sufficient quantity for therapeutic use can be obtained, but the production process becomes very complicated
Solution Approach 1:
The patent uses enzymatic reactions as an intermediary process to convert polyunsaturated fatty acids into SPM. Instead of direct organic synthesis, enzymes act as catalysts to facilitate the transformation, simplifying the production process while maintaining sufficient product quantity.
Solution Approach 2:
The patent replaces complex mechanical/chemical synthesis processes with biochemical enzymatic reactions. This substitution uses biological catalysts (enzymes) to achieve the same chemical transformation with simpler, more controlled conditions, reducing overall process complexity.
2Reliability
If conventional anti-inflammatory agents are used, then production of inflammatory signal substances can be inhibited, but they cannot effectively terminate the inflammatory response
Solution Approach 1:
The patent changes the chemical parameters of fatty acid derivatives by introducing specific hydroxyl groups at defined positions and configurations. This parameter modification transforms ordinary fatty acid derivatives into specialized pro-resolving mediators with enhanced ability to terminate inflammatory responses, improving therapeutic efficacy.
3Quantity of substance
If multiple enzymes are used continuously to produce SPM in vivo, then sufficient SPM can be produced, but the production amount remains very trace
Solution Approach 1:
The patent performs preliminary concentration of polyunsaturated fatty acids before enzymatic conversion. By pre-concentrating the substrate and optimizing enzyme reaction conditions, the process achieves much higher productivity and SPM yield compared to trace in vivo production, while maintaining the benefits of enzymatic catalysis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The enzyme effectively produces mono- or di-hydroxy derivatives, enhancing collagen synthesis, reducing inflammation, and improving skin barrier function, effectively addressing skin aging and atopic dermatitis.
Implementation Method 1
An enzyme with a specific amino acid sequence is used to produce mono- or di-hydroxy derivatives of polyunsaturated fatty acids
Data Source
AI summary
The present invention relates to an enzyme for producing hydroxy derivatives of polyunsaturated fatty acids, a method for producing hydroxy derivatives of polyunsaturated fatty acids using the same, and an SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids.The enzyme may produce hydroxy derivatives of polyunsaturated fatty acids in a single reaction, and thus may be very usefully used for in vitro production. In addition, the SPMs complex composition containing the hydroxy derivatives of polyunsaturated fatty acids promotes collagen production, inhibits the production of NO and expression of TNF-α and IL-6, increases the expression of filaggrin and loricrin, and thus may be usefully used for preventing or alleviating skin aging or wrinkles, strengthening skin barrier, or preventing, alleviating or treating skin diseases comprising atopic dermatitis.