Carbonate-Substituted Hydroxyapatite Carrier for Plant Vascular Pathogens

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Solution Overview

Problem

Current methods are inadequate for effectively controlling vascular diseases in plants such as grapevine yellows, bacterial canker in kiwifruit, olive quick decline syndrome, and citrus bacterial canker, due to the difficulty in reaching and treating pathogens within plant vascular tissues.

Innovation Solution

The use of carbonate-substituted hydroxyapatite as a carrier for bioactive substances, which penetrates plant tissues through natural openings, allowing for intimate contact with pathogens and enhanced release of anti-parasite and nutritional agents.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional bioactive substances are applied to plants, then they remain on the surface, but they cannot reach pathogens within vascular tissues

Engineering Contradiction:
Improveefficacy of pathogen treatmentVSAvoiddelivery to target site
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

Hydroxyapatite particles serve as intermediary carriers that transport bioactive substances from the plant surface through natural openings (stomata, lenticels, wounds) into the vascular tissues where pathogens reside. The particles act as a delivery vehicle that bridges the gap between external application and internal target sites.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The hydroxyapatite particles provide a porous matrix structure that allows bioactive substances to be loaded, protected, and gradually released. The porous nature facilitates penetration through plant tissues and enables sustained delivery of anti-parasite agents to the vascular system.

Inventive Principle:
Principle #31Porous materials

2Reliability

If bioactive substances are applied at high concentrations, then pathogen contact is enhanced, but substance loss and environmental impact increase

Engineering Contradiction:
Improvepathogen contact effectivenessVSAvoidbioactive substance stability
Core Design Contradiction:
ReliabilityVSLoss of substance

Solution Approach 1:

The hydroxyapatite carrier changes the delivery parameters of bioactive substances by enabling localized concentration at the pathogen site while reducing overall application rates. The controlled release mechanism maintains effective concentrations where needed without excessive environmental dispersion.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The combination of hydroxyapatite with bioactive substances creates a composite material system where the inorganic carrier enhances the stability and targeted delivery of organic anti-parasite agents, reducing degradation and off-target losses.

Inventive Principle:
Principle #40Composite materials

3Reliability

If vascular diseases are treated early, then disease progression is halted, but detection difficulty prevents timely intervention

Engineering Contradiction:
Improvedisease control timingVSAvoidpathogen localization
Core Design Contradiction:
ReliabilityVSDifficulty of detecting and measuring

Solution Approach 1:

The hydroxyapatite particles can be functionalized with color indicators or fluorescent markers that enable visual or instrumental detection of particle localization within plant tissues. This allows monitoring of delivery effectiveness and pathogen contact without requiring complex diagnostic procedures.

Inventive Principle:
Principle #32Color changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The carbonate-substituted hydroxyapatite effectively delivers bioactive substances to the site of infection, reducing disease symptoms and improving plant health by targeting pathogens within the plant's vascular system.

Implementation Method 1

The carbonate-substituted hydroxyapatite carrier loaded with at least one bioactive substance... penetrates into the plants through natural openings... thus realizing an intimate contact with pathogen cells

Methodology Applied
Scientific EffectAdsorption: Adsorption

Implementation Method 2

enhanced release of anti-parasite and nutritional agents

Methodology Applied
Scientific EffectDesorption: Desorption

Data Source

PatentEP3297432B1Use of hydroxyapatite as a carrier of bioactive sustances for treating vascular diseases in plants
Publication Date: 2022.06.22 NDG NATURAL DEVELOPMENT GROUP SRL
  • EP3297432B1 patent drawingFigure 1A
  • EP3297432B1 patent drawingFigure 1B
  • EP3297432B1 patent drawingFigure 1C

AI summary

The subject matter of the invention is a hydroxyapatite carrier, corresponding to the formula : Ca(10-x)Mx(PO4)(6-y)(CO3)y(OH)2 in which: M is chosen from Cu, Mg, Zn, Pb, Cd, Ba, K, Mn, Mo, Fe, Ag, B, Se or a combination of at least two of such elements; - x is comprised between 0 and 2, preferably between 0 and 1, more preferably between 0 and 0.8, even more preferably between 0.02 and 0.05; - y is comprised between 0 and 2, preferably between 0 and 1, more preferably between 0.002 and 0.030, The hydroxyapatite is a carrier of a bioactive substance chosen among a metal ion selected from Cu, Zn, S, Mn, Mg, K, Fe, B, Mo, Se and/or an extract of vegetal origin. The bioactive substances are active in the treatment of vascular diseases of plants, in particular: grapevine yellows (caused by grapevine phytoplasma), bacterial canker in kiwifruit (caused by Pseudomonas syringae), olive quick decline syndrome (caused by Xylella fastidiosa) and Citrus bacterial canker disease (caused by Xanthomonas axonopodis).