Hyperbaric Cryogenesis Chamber for Cell Viability and Proliferation
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Solution Overview
Problem
Current technologies lack a method to induce cellular proliferation or nonproliferation of tumoral cells in an extracorporeal, organized manner while maintaining cellular integrity under intermittent pressure and cold temperature conditions with physiological fluids.
Innovation Solution
A hyperbaric cryogenesis chamber that combines cryopreservation and hyperbaric oxygen therapy by pressurizing and depressurizing compartments with gases like pure oxygen, allowing for controlled temperature changes and gas mixtures to induce cellular reproduction and nonproliferation of tumoral cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cryopreservation is used to preserve cells and tissues, then cell viability is maintained, but cellular reproduction is not induced
Solution Approach 1:
The patent applies periodic action by subjecting cells to alternating cycles of hyperbaric oxygenation and cryopreservation. During hyperbaric oxygenation phases, cells are exposed to high oxygen pressure to stimulate proliferation, then rapidly cooled during cryopreservation phases to maintain viability and prevent tumoral cell growth. This periodic alternation between opposing conditions enables both cell reproduction and viability maintenance.
2Productivity
If hyperbaric oxygenation is used to accelerate cellular reproduction, then cell proliferation is stimulated, but tumoral cells may also proliferate
Solution Approach 1:
The patent uses periodic alternation between hyperbaric oxygenation and rapid cryopreservation to stimulate desired cell proliferation while preventing tumoral cell growth. The brief exposure periods to hyperbaric oxygen stimulate reproduction of normal cells, while the subsequent rapid cooling phases induce apoptosis in tumoral cells, thereby achieving selective proliferation control.
Solution Approach 2:
The patent employs strong oxidant effects during hyperbaric oxygenation phases, where cells are exposed to high concentrations of oxygen under pressure. This accelerated oxidation stimulates cellular metabolism and proliferation of normal cells. The same oxidant effect, when combined with subsequent cryopreservation, contributes to tumoral cell destruction through oxidative stress damage.
3Reliability
If rapid cooling is applied to preserve cells, then cell integrity is maintained, but cellular metabolism is halted
Solution Approach 1:
The patent implements periodic cycles where rapid cooling for integrity preservation is alternated with hyperbaric oxygenation phases that restore metabolism. During cooling phases, cell integrity is maintained through cryopreservation, while during subsequent hyperbaric oxygenation phases, cellular metabolism is reactivated and stimulated, preventing permanent metabolic halt.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This combination accelerates cellular reproduction and nonproliferation of tumoral cells, extending cell viability and potentially aiding in treating terminal pathologies like leukemia and hepatopathology, while also preserving tissues and organs for longer periods.
Implementation Method 1
pressurizing and depressurizing compartments with gases like pure oxygen, allowing for controlled temperature changes and gas mixtures to induce cellular reproduction and nonproliferation of tumoral cells
Implementation Method 2
The hyperbaric cryogenesis chamber can have in internal or external hermetic system in order to lower the environment temperature
Implementation Method 3
The tubing that is preferably made of copper is the tubing which allows the circulation of refrigerating gas in its inside
Data Source
AI summary
The Hyperbaric Cryogenesis Chambers are equipment for medical usage, extracorporeal, and capable of promoting the proliferation and preservation of cells in a way mainly microbiologic, “not biologic”. They are compartments with containers that host tissues or cells in solution with nutrients, that bear pressures higher and lower to sea level. They have a cold system inside that lowers the environmental temperature and maintains it permanently. They are for maintaining viability of cells or tissues and to induce their organized proliferation with oxygen or other gases at pressures higher or lower that the exterior of the chamber.