Hyperimmunized Egg Product for Necrotic Enteritis Control

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Solution Overview

Problem

Necrotic enteritis in poultry production, caused by Clostridium perfringens, poses significant economic losses due to mortality and decreased growth performance, and the increasing resistance to antibiotics necessitates the development of non-antibiotic strategies for control and prevention.

Innovation Solution

A method involving the administration of a hyperimmunized egg product containing antibodies to specific antigens such as Clostridium perfringens toxins and Eimeria proteins to avian species, which are produced by hyperimmunizing egg-producing animals with antigens like Clostridium perfringens α-toxin, EF-Tu, NetB toxin, PFO, and Eimeria proteins, resulting in a therapeutically effective IgY antibody content.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are used for treatment of necrotic enteritis, then disease control and prevention is effective, but antibiotic resistance develops and human health risks increase

Engineering Contradiction:

Improvedisease control effectivenessVSAvoidantibiotic resistance and human health risks

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses IgY antibodies as an intermediary substance to neutralize Clostridium perfringens toxins. These antibodies are produced by hyperimmunizing egg-laying hens with C. perfringens antigens, then collecting IgY from their eggs. The IgY antibodies serve as a mediator that binds to and neutralizes bacterial toxins in the poultry gut, providing disease protection without using antibiotics, thus avoiding antibiotic resistance issues while maintaining effective disease control

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates a biological copy of the immune response by producing IgY antibodies in hens that mirror the protective immunity needed in poultry. The hyperimmunized hens generate antibodies that are then transferred through eggs to provide passive immunity to broiler chickens, effectively copying the protective immune mechanism without requiring direct antibiotic treatment of the broilers

Inventive Principle:

Principle #26Copying

2Object-affected harmful factors

If hyperimmunized egg product is administered, then necrotic enteritis is prevented or treated without antibiotic resistance, but the complexity of antibody production and egg collection increases

Engineering Contradiction:

Improveantibiotic resistance avoidanceVSAvoidhyperimmunization process complexity

Core Design Contradiction:

Object-affected harmful factorsVSDevice complexity

Solution Approach 1:

The system uses egg-laying hens as self-service antibody production factories. The hens are hyperimmunized once with C. perfringens antigens, and then they continuously produce IgY antibodies that are secreted into their eggs. This self-service mechanism eliminates the need for repeated antibody extraction or complex bioreactor systems, as the hens automatically generate and export antibodies through their natural egg-laying process

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The hyperimmunized hens serve multiple functions: they act as antigen recipients during immunization, as antibody production factories, and as automated extraction systems through egg collection. This multi-functionality consolidates several complex steps into a single biological system, simplifying the overall process while maintaining effective antibody production for necrotic enteritis prevention

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The hyperimmunized egg product effectively prevents or treats necrotic enteritis by providing elevated antibody levels, reducing disease severity and economic losses, while avoiding antibiotic resistance issues.

Implementation Method 1

hyperimmunizing the egg-producing animal with the antigen

Methodology Applied

Scientific EffectHyperimmunization:

Implementation Method 2

the hyperimmunized egg product comprises a therapeutically effective amount of an antibody to an antigen selected from the group consisting of Clostridium perfringens α-toxin, Clostridium perfringens elongation factor Tu (EF-Tu), Clostridium perfringens necrotic enteritis B-like (NetB) toxin, Clostridium perfringens Pyruvate: Ferredoxin oxidoreductase (PFO)

Methodology Applied

Scientific EffectAntibody production:

Implementation Method 3

administering to the avian a therapeutically effective amount of a hyperimmunized egg product... thereby preventing or treating the necrotic enteritis in the subject

Methodology Applied

Scientific EffectAntibody-antigen binding:

Data Source

PatentUS11230590B2Hyperimmunized egg product for treatment of necrotic enteritis in poultry

Publication Date: 2022.01.25 ARKION LIFE SCIENCES LLC

AI summary

In one aspect, the present invention is directed to a method for preventing or treating necrotic enteritis by administering a hyperimmunized egg product obtained from an egg-producing animal to an avian. The hyperimmunized egg product may contain an antibody specific to an antigen selected from the group consisting of Clostridium perfringens α-toxin, Clostridium perfringens elongation factor Tu (EF-Tu), Clostridium perfringens necrotic enteritis B-like (NetB) toxin, Clostridium perfringens Pyruvate: Ferredoxin oxidoreductase (PFO), and Eimeria tenella elongation factor 1-alpha.