Hyperspectral Optical Platform for Small-Volume Multi-Property Analysis
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Solution Overview
Problem
Existing methods for characterizing the physicochemical properties of pharmaceutical compositions, such as viscosity and aggregation, require large sample volumes and long analysis times, leading to material waste and low-throughput analysis.
Innovation Solution
A high-throughput optical system and platform utilizing a spectral/hyperspectral imaging device that simultaneously assesses viscosity and additional properties like aggregation and phase separation, using environmentally sensitive fluorophores and fluorescent tracker particles, capable of analyzing small sample volumes in seconds.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional characterization methods are used to assess multiple physicochemical properties, then measurement precision is achieved, but sample volume requirement increases and analysis time increases
Solution Approach 1:
The patent combines multiple characterization techniques (flow cytometry, microscopy, spectroscopy) into a single integrated optical platform that simultaneously assesses multiple physicochemical properties including viscosity, aggregation, phase separation, pH, and ionic strength from a single small sample volume, eliminating the need for separate assays
Solution Approach 2:
The optical platform is designed as a universal system that can measure multiple different physicochemical properties using the same instrument and small sample volume, with the system adapting its detection parameters to characterize different properties such as viscosity through particle dynamics and aggregation through scattering patterns
2Measurement precision
If traditional characterization methods are used to assess multiple physicochemical properties, then measurement precision is achieved, but analysis time increases
Solution Approach 1:
The patent merges multiple separate characterization assays into a single simultaneous measurement process, where viscosity, aggregation, phase separation, pH, and ionic strength are all assessed in parallel from the same sample, reducing total analysis time compared to sequential testing
Solution Approach 2:
The optical platform enables continuous monitoring and rapid data acquisition without interruption, allowing multiple properties to be measured in a continuous flow manner rather than through discrete sequential steps, thereby reducing overall analysis time
3Measurement precision
If traditional characterization methods are used, then measurement precision is achieved, but productivity decreases
Solution Approach 1:
The patent integrates multiple characterization capabilities into one platform that can process multiple samples simultaneously or sequentially with minimal preparation time, significantly increasing throughput compared to traditional methods that require separate assays for each property
Solution Approach 2:
The system rapidly adjusts detection parameters and measurement modes to characterize different physicochemical properties without requiring physical reconfiguration or extensive recalibration, enabling high-speed switching between viscosity measurement, aggregation analysis, and other property assessments to maximize productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid assessment of multiple properties in small volumes, reducing material waste and increasing throughput, facilitating faster development of stable biomolecule-based therapeutics.
Implementation Method 1
a spectral/hyperspectral imaging device that can simultaneously assess environmentally sensitive fluorophore probe emission spectra
Implementation Method 2
environmentally sensitive fluorophore probe emission spectra
Implementation Method 3
track the dynamics of one or more fluorescent tracker particle (nano- and micro-) dynamics
Data Source
AI summary
Disclosed herein are optical systems and platforms for the simultaneous assessment of one or more properties of a sample, e.g., a biomolecule-containing pharmaceutical composition, including, e.g., aggregation, phase separation, and/or viscosity, as well as methods of using the same.