Iceberg Lettuce Breeding for Uniform Fusarium-Resistant Bison
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Solution Overview
Problem
Existing lettuce breeding methods struggle to produce uniform varieties with desirable traits such as resistance to pests and diseases, while maintaining consistent performance and yield, due to genetic non-uniformity in hybrid plants.
Innovation Solution
Development of the iceberg lettuce cultivar Bison through pedigree selection and backcrossing techniques, combined with genetic transformation, to introduce specific traits like herbicide tolerance and disease resistance, ensuring genetic uniformity and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If cross-pollination is used to combine desirable traits from different varieties, then genetic diversity and trait variety are improved, but genetic uniformity and performance predictability deteriorate
Solution Approach 1:
The breeding process is segmented into distinct phases: first developing uniform inbred lines through self-pollination, then crossing these standardized lines to create predictable hybrids. This segmentation allows genetic diversity to be introduced in a controlled manner while maintaining uniformity within each parental line, thereby ensuring performance predictability in the resulting hybrid population.
Solution Approach 2:
The patent changes the genetic parameter state by transitioning from heterozygous cross-pollinated plants to homozygous inbred lines through multiple generations of self-pollination. This parameter change (from heterozygosity to homozygosity) ensures genetic uniformity within lines, which when crossed, produces uniform hybrid progeny with predictable performance characteristics.
2Reliability
If self-pollination is used to develop uniform varieties, then genetic uniformity is improved, but genetic diversity and trait combination capability deteriorate
Solution Approach 1:
The patent applies preliminary action by first developing uniform inbred lines through extended self-pollination before the crossing stage. This preliminary development of genetic uniformity in parental lines ensures that when crossing occurs, the resulting hybrids will exhibit consistent and predictable performance, as both parents contribute uniform genetic material rather than heterogeneous genomes.
Solution Approach 2:
The patent extracts the genetic uniformity characteristic from the self-pollination process and applies it specifically to the development of parental inbred lines. By taking out and isolating this uniformity trait in the parental generation, the method ensures that subsequent hybrid crosses will produce uniform progeny, while still allowing for genetic diversity to be introduced through the selection of different inbred line combinations.
3Reliability
If multiple generations of inbreeding are performed to develop homozygous lines, then genetic uniformity is improved, but breeding time and complexity increase
Solution Approach 1:
The patent employs self-service through self-pollination, where each plant fertilizes itself to produce the next generation. This self-service mechanism naturally progresses the inbreeding process without requiring complex external intervention, allowing multiple generations of inbreeding to occur systematically. By using the plant's own reproductive system, the method achieves homozygosity efficiently while minimizing the need for additional breeding infrastructure or complex manual pollination procedures.
Data Source
AI summary
The invention provides seed and plants of the iceberg lettuce cultivar designated cv. Bison. The invention thus relates to the plants, seeds and tissue cultures of iceberg lettuce cv. Bison, and to methods for producing a lettuce plant produced by crossing a plant of iceberg lettuce cv. Bison with itself or with another lettuce plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of iceberg lettuce line cv. Bison, including the gametes of such plants.