IGFBP Biomarker Analysis for Short- vs Long-Term Animal Welfare

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Solution Overview

Problem

Existing methods fail to effectively differentiate between short-term and long-term impairments in animal welfare, particularly during animal transport, using biomarkers.

Innovation Solution

Analyzing the expression of IGFBP-2 and IGFBP-3 in animal samples, such as blood or meat, to determine the impact of transport stress on animal welfare, with a reduced ratio of IGFBP-3 to IGFBP-2 indicating long-term impairment and low expression of both indicating short-term impairment.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If existing biomarker methods are used to assess animal welfare, then general welfare status can be determined, but differentiation between short-term and long-term impairments is not possible

Engineering Contradiction:
Improvewelfare impairment differentiationVSAvoidtemporal welfare information
Core Design Contradiction:
Measurement precisionVSLoss of information

Solution Approach 1:

The patent segments the welfare assessment into two distinct temporal categories by measuring two different biomarkers: IGFBP-2 for short-term stress (transport) and IGFBP-3 for long-term welfare status. This segmentation allows simultaneous detection of both acute and chronic welfare conditions, resolving the contradiction between general assessment capability and temporal differentiation precision.

Inventive Principle:
Principle #1Segmentation

2Productivity

If transport time is extended to meet logistical requirements, then productivity is improved, but animal welfare deteriorates due to increased stress

Engineering Contradiction:
Improvetransport efficiencyVSAvoidtransport stress
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent implements a feedback mechanism where IGFBP-2 and IGFBP-3 levels are measured to provide information about the actual welfare impact of transport duration. This feedback allows optimization of transport logistics by identifying the threshold where welfare deterioration begins, enabling productivity improvements without exceeding welfare-critical transport times.

Inventive Principle:
Principle #23Feedback

3Object-affected harmful factors

If transport conditions are strictly optimized for animal welfare, then welfare is improved, but productivity and transport efficiency deteriorate

Engineering Contradiction:
Improvetransport stressVSAvoidtransport efficiency
Core Design Contradiction:
Object-affected harmful factorsVSProductivity

Solution Approach 1:

The patent changes the assessment parameters from binary welfare judgments to quantitative biomarker measurements (IGFBP-2 and IGFBP-3 concentrations). This allows identification of optimal transport parameter ranges (duration, conditions) that maintain welfare above critical thresholds while maximizing productivity, resolving the contradiction between welfare optimization and transport efficiency.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3335049B1Analysis of igf-binding proteins for determination of animal welfare
Publication Date: 2026.03.11 FORSCHUNGSINSTITUT FUER NUTZTIERBIOLOGIE
  • EP3335049B1 patent drawingFigure 1(A)~1(C)
  • EP3335049B1 patent drawingFigure 2(A)~2(D)
  • EP3335049B1 patent drawingFigure 3

AI summary

The invention relates to a method and kit for determining animal welfare, also animal wellbeing. In particular, the invention relates to a method for analysing the expression of IGF (Insulin-like growth factor) binding protein, in particular IGFBP-2 and IGFBP-3, as a marker for animal welfare. Said method is different due to the examination of said marker between short-term and long-term impairment of the welfare of the animal, i.e. for example between impairments, the short-term for example when transporting animals, or impairments which occur due to illness and/or due to inadequate conditions in which animals are kept.