Multiple-variable IL-2 Dose Regimen for GVHD

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Solution Overview

Problem

Current treatments for graft-versus-host disease (GVHD) are inadequate in maintaining long-term inflammatory control and often fail to effectively enhance the function of adoptively transferred regulatory T-cells (Tregs), particularly in pediatric patients, who suffer from debilitating consequences such as sclerotic skin and pulmonary fibrosis.

Innovation Solution

A multiple-variable dose regimen of Interleukin-2 (IL-2) is administered to pediatric patients, first as an induction regimen to increase the ratio of regulatory T lymphocytes (Tregs) to conventional T lymphocytes (Tcons), followed by a maintenance regimen with a higher dose to further enhance Treg numbers and activity, potentially combined with additional therapies like ECP or Treg infusions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If low-dose IL-2 is administered to enhance Tregs, then Treg function is improved, but clinical benefit is obtained in only half of patients

Engineering Contradiction:
Improveclinical benefit rateVSAvoidresponse variability
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent implements dynamic dosing by transitioning from a fixed low-dose IL-2 regimen to a multi-phase protocol where the dose is adjusted based on patient response and Treg:Tcon ratio measurements. The induction phase uses higher doses to rapidly expand Tregs, followed by maintenance phases with adjusted doses to sustain therapeutic effect, thereby improving reliability while reducing response variability.

Inventive Principle:
Principle #15Dynamics

Solution Approach 2:

The patent incorporates feedback mechanisms by monitoring Treg:Tcon ratios and clinical response to adjust IL-2 dosing. Phase 1 establishes baseline Treg expansion, phase 2 adjusts dosing based on achieved ratios, and phase 3 maintains therapy based on sustained response, creating a closed-loop system that improves clinical benefit rate and reduces variability.

Inventive Principle:
Principle #23Feedback

2Reliability

If ex vivo expanded Tregs are infused to control immune activation, then immune tolerance is improved, but long-term function maintenance is difficult to achieve

Engineering Contradiction:
Improvelong-term immune controlVSAvoidTreg function duration
Core Design Contradiction:
ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent applies preliminary action by using the induction phase to pre-expand and activate Tregs before they need to provide long-term control. The high-dose IL-2 in phase 1 creates a robust Treg pool that can then be maintained at lower doses in phases 2 and 3, ensuring long-term function is established before maintenance begins.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent ensures continuity of useful action by implementing a multi-phase continuous IL-2 therapy protocol. Rather than a single infusion, the treatment continues through induction, consolidation, and maintenance phases with ongoing IL-2 administration that sustains Treg function and prevents decay over time, thereby achieving long-term immune control.

Inventive Principle:
Principle #20Continuity of useful action

3Reliability

If a single fixed dose of IL-2 is used to treat GVHD, then treatment simplicity is maintained, but inadequate clinical response is observed in many patients

Engineering Contradiction:
Improveclinical response rateVSAvoiddosing regimen complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the treatment into distinct phases (induction, consolidation, maintenance) with different dosing strategies. Phase 1 uses higher doses for rapid Treg expansion, phase 2 adjusts based on response, and phase 3 maintains therapy. This segmentation improves clinical response by addressing different therapeutic needs at different time points while organizing complexity into manageable stages.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes dosing parameters over time and based on patient response. The IL-2 dose is adjusted between phases and within phases based on Treg:Tcon ratios and clinical status. This dynamic parameter adjustment improves clinical response rate by optimizing the balance between efficacy and toxicity for each patient, while the structured approach manages the inherent complexity.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3204119B1Multiple-variable il-2 dose regimen for treating immune disorders
Publication Date: 2021.06.09 DANA FARBER CANCER INSTITUTE INC
  • EP3204119B1 patent drawingFigure 1~2
  • EP3204119B1 patent drawingFigure 3~4
  • EP3204119B1 patent drawingFigure 5

AI summary

The present invention is based, in part, on the identification of iiicihods of using multiple-variable IL-2 doses for identifying, assessing, preventing, and treating immune disorders.