IL-15 Fusion Protein Complexes for Immune Stimulation

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

There is a need for new strategies to augment immune responses and provide therapeutic benefits for patients with neoplasia or infectious diseases, as existing approaches are insufficient in effectively stimulating immune cells and targeting disease cells.

Innovation Solution

The development of multi-specific interleukin-15 (IL-15)-based fusion protein complexes that incorporate IL-12 and IL-18 binding domains, specifically an IL-15N72D:IL-15RαSu-Ig Fc scaffold fused to these binding domains, which enhance immune cell stimulation and activity against disease cells.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If multiple individual cytokines are used to stimulate immune cells, then immune cell activity is enhanced, but the complexity of administration and delivery increases

Engineering Contradiction:

Improveimmune cell stimulation efficacyVSAvoidcomplexity of cytokine combination administration

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent combines multiple cytokine binding domains (IL-12, IL-15, IL-18) into a single fusion protein complex, merging the functions of multiple individual cytokines into one molecular entity that can be administered as a single therapeutic agent while maintaining the ability to stimulate multiple immune cell pathways

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The fusion protein complex is designed with multiple cytokine binding domains that enable it to interact with different cytokine receptors (IL-12R, IL-15R, IL-18R) on immune cells, providing multi-functional immune stimulation through a single molecule that acts as a universal immune activator

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Reliability

If fusion protein complexes with multiple binding domains are developed, then binding affinity to target antigens is enhanced, but the manufacturing complexity increases

Engineering Contradiction:

Improvebinding affinity to target antigensVSAvoidmanufacturing complexity of fusion protein complex

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The fusion protein complex is constructed by segmenting different cytokine binding domains (IL-12, IL-15, IL-18) and assembling them into a modular framework, allowing each domain to be independently optimized and the overall structure to be manufactured using standardized protein expression and purification protocols

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent creates a composite protein structure by fusing multiple cytokine binding domains into a single polypeptide chain, combining the functional properties of different cytokine receptors into one molecule that can be produced as a single recombinant protein product

Inventive Principle:

Principle #40Composite materials

Data Source

PatentUS20250152686A1Il-15-based fusions to il-12 and il-18

Publication Date: 2025.05.15 ALTOR BIOSCIENCE LLC

AI summary

The invention features multi-specific fusion protein complexes with one domain comprising IL-15 or a functional variant and a binding domain specific to IL-12 or IL-18.