IMAC Stationary Phase for Natural Metal Ion Complexing
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Solution Overview
Problem
Oxidative degradation reactions in orally consumable preparations, particularly foods, lead to sensory instability due to the involvement of Ni2+, Cu2+, and Fe2+ ions, causing off-flavors and aroma defects, which existing antioxidants like EDTA can exacerbate by strongly complexing metal ions, necessitating a natural substance that can form complexes with these ions without excessive binding.
Innovation Solution
A method involving immobilized metal ion affinity chromatography (IMAC) using a stationary phase loaded with Ni2+, Cu2+, and Fe2+ ions to identify and isolate natural substances from plant extracts that can form complexes with these ions, specifically focusing on non-protein compounds with molecular weights less than 1000 g/mol, which are suitable for human consumption and sensory stabilization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If EDTA or EDTA salts are used to complex metal ions, then metal ion complexing effect is enhanced, but metal ions are excessively depleted from the human body
Solution Approach 1:
The patent changes the parameter of complexing strength by selecting natural substances with moderate metal ion complexing capabilities, unlike EDTA which has excessively strong complexing effects. This resolves the contradiction by finding a balance between sensory stabilization and avoiding metal ion depletion.
Solution Approach 2:
The patent uses natural substances that are suitable for human consumption rather than synthetic agents like EDTA. These natural substances provide the necessary complexing effect temporarily for sensory stability without the harmful long-term effects of metal ion depletion.
2Reliability
If antioxidants are used to prevent oxidative degradation, then sensory stability is improved, but off-flavors and aroma defects still occur due to metal ion catalysis
Solution Approach 1:
The patent introduces natural substances as intermediary compounds that specifically complex with metal ions (Ni2+, Cu2+, Fe2+) to prevent their catalytic activity. This intermediary action blocks the harmful oxidative degradation pathway while avoiding the excessive complexing effects of EDTA.
Solution Approach 2:
The patent combines antioxidants with natural metal ion complexing substances to create a composite stabilization system. This composite approach addresses both oxidative degradation and metal ion catalysis simultaneously, preventing off-flavors more effectively than antioxidants alone.
3Reliability
If strong metal ion complexing agents like EDTA are used, then oxidative degradation is reduced, but the substances cannot be classified as naturally consumable
Solution Approach 1:
The patent selects natural substances that are suitable for human consumption and of natural origin, replacing synthetic agents like EDTA. These natural substances provide adequate metal ion complexing for sensory stability while maintaining adaptability for human consumption and natural origin classification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method selectively enriches and identifies natural substances that can complex with Ni2+, Cu2+, and Fe2+ ions, reducing their catalytic activity and oxidative degradation, thereby enhancing the sensory stability of food products without the adverse effects of strong metal ion complexing agents like EDTA.
Implementation Method 1
a natural product-containing extract is passed over a stationary phase loaded with Ni2+, Cu2+, and/or Fe2+ ions, which is suitable for immobilized metal ion affinity chromatography (IMAC)
Implementation Method 2
the natural product capable of complex formation with Ni2+, Cu2+, and/or Fe2+ ions is adsorbed onto the stationary phase
Implementation Method 3
These natural substances should in particular reduce or prevent the catalytic activity of any metal ions present and oxidative degradation reactions
Implementation Method 4
Oxidative degradation reactions of certain components in orally consumed preparations, especially foods, can frequently lead to changes in aroma and/or taste over time
Data Source
AI summary
Identifying natural substance that forms complex with nickel(II), copper(II) and/or iron(II) ions, comprises e.g. (a) providing an aqueous, alcoholic or aqueous-alcoholic natural substance containing extract, (b) providing a device comprising a stationary phase loaded with nickel(II), copper(II) and/or iron(II) ions, which is suitable for immobilized metal ion affinity chromatography (IMAC), and (c) passing natural substance containing extract obtained in step (a) through device of (b) for carrying out the adsorption of the natural substance at the stationary phase of the IMAC device. Identifying natural substance that forms complex with nickel(II), copper(II) and/or iron(II) ions, comprises (a) providing an aqueous, alcoholic or aqueous-alcoholic natural substance containing extract, (b) providing a device comprising a stationary phase loaded with nickel(II), copper(II) and/or iron(II) ions, which is suitable for immobilized metal ion affinity chromatography (IMAC), (c) passing the natural substance containing extract obtained in step (a) through the device of (b) for carrying out the adsorption of the natural substance at the stationary phase of the IMAC device, (d) optionally flushing the natural substance loaded stationary phase of the IMAC device with a solvent, (e) desorbing the natural substance from the stationary phase of the IMAC device, by passing a solution containing at least one desorbent through the IMAC device, (f) optionally separating the natural substance from desorbent, and (g) identifying the natural substance that forms complex with nickel(II), copper(II) and/or iron(II) ions. An independent claim is also included for the use of nickel(II), copper(II) and/or iron(II) ions loaded stationary phase that is suitable for immobilized metal ion-affinity-chromatography, for identifying a natural substance that forms complex with nickel(II), copper(II) and/or iron(II) ions.