Immortalized humanized sebaceous gland precursor cell, preparation method therefor, and method for screening and evaluating grease regulation drug or skin care product by using sebaceous gland precursor cell
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Solution Overview
Problem
Current sebaceous gland cell lines, such as hamster and human SZ95, are unsuitable for genetic research due to unclear genome information, lack of androgen receptors, and potential malignant transformation, limiting their ability to replicate sebaceous gland behaviors in acne patients, and existing human cells have limited expansion and heterogeneity issues.
Innovation Solution
An immortalized human sebaceous gland progenitor cell line derived from urine-derived hiPSCs is developed, using a specific culture and differentiation method to achieve high purity and yield, enabling stable and accurate lipid secretion and droplet formation, suitable for screening and evaluating oil-regulating drugs or skincare products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If hamster sebaceous gland cell line is used, then liquid droplet formation ability is maintained, but genome information clarity and suitability for genetic research deteriorates
Solution Approach 1:
The patent creates a human-derived cell line (SZ95) that copies the essential functional characteristics of sebaceous gland cells, including liquid droplet formation ability, while maintaining complete and clear human genome information. This allows genetic research to be conducted without compromising the reliability of sebaceous gland-specific functions.
2Loss of information
If human sebaceous gland cell line SZ95 is used, then genome information clarity and proliferative capacity are improved, but androgen receptor presence and response to testosterone deteriorates
Solution Approach 1:
The patent identifies and addresses the parameter deficiency in SZ95 cells by supplementing the culture system with androgen receptors or testosterone-responsive components, enabling the cells to respond to testosterone stimulation while maintaining their clear genome information and high proliferative capacity.
3Quantity of substance
If sebaceous gland cells are isolated from multiple donors, then cell quantity is increased, but heterogeneity and research outcome stability deteriorates
Solution Approach 1:
The patent establishes a standardized cell line with consistent genetic background and uniform characteristics that can be propagated indefinitely. This homogeneous cell source provides large quantities of cells while maintaining research outcome stability, eliminating the variability introduced by isolating cells from multiple donors.
4Quantity of substance
If sebaceous gland cells are primarily cultured, then lipid accumulation in cytoplasm is achieved, but cell generation limit and expansion capability deteriorates
Solution Approach 1:
The patent creates an immortalized cell line that possesses inherent self-renewal capabilities through integrated viral oncogenes, allowing the cells to bypass normal generation limits. These cells can continuously proliferate and maintain lipid accumulation capacity without requiring periodic isolation from new donors.
Data Source
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AI summary
The present disclosure provides an immortalized human sebaceous gland progenitor cell deposited at the China Center for Type Culture Collection (CCTCC) under an accession number of CCTCC NO: C2023286. The present disclosure also provides a preparation method and use of the immortalized human sebaceous gland progenitor cell. The immortalized human sebaceous gland progenitor cell can be directly used for screening and evaluating oil-regulating drugs or skincare products. Alternatively, the immortalized human sebaceous gland progenitor cell can further differentiate into a mature sebaceous gland cell, and the mature sebaceous gland cell is then utilized for screening and evaluating oil-regulating drugs or skincare products. The present disclosure has the following advantage: The sebaceous gland progenitor cell and the mature sebaceous gland cell are stable and accurate, and the expression of genes related to liquid droplet formation, lipid storage, and androgen receptor (AR) in these two cells is significantly enhanced. As a result, these two cells are highly suitable for screening and evaluation of oil-regulating drugs or skincare products.