Immune Cell Cryopreservation Medium Using Albumin and Disaccharides
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Solution Overview
Problem
Cryopreservation of living cells, particularly immune cells, is challenging due to the harsh conditions imposed by freezing and thawing, leading to low survivability and viability rates.
Innovation Solution
A cryopreservation medium comprising a non-pyrogenic and isotonic crystalloid solution, a disaccharide, a cryoprotectant, and human serum albumin is used to enhance the survivability and viability of immune cells during freezing and thawing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If cells are subjected to freezing and thawing conditions, then cell preservation is achieved, but cell survivability and viability rates are reduced
Solution Approach 1:
The patent modifies the chemical composition parameters of the freezing medium by incorporating specific protective agents (albumin, disaccharides, cryoprotectants) to change the physical-chemical environment during freezing, thereby reducing cellular damage while maintaining preservation duration
Solution Approach 2:
The invention uses a composite cryopreservation medium containing multiple components (crystalloid solution, albumin, disaccharides, and cryoprotectants) that work synergistically to protect cells during freezing and thawing, resolving the contradiction between preservation duration and cell viability
2Stability of the object's composition
If extracellular medium freezes, then osmotic equilibrium is maintained, but intracellular water loss and solute concentration increase occur
Solution Approach 1:
The patent introduces albumin and disaccharides as intermediary substances that mediate between the extracellular freezing environment and the intracellular space, preventing direct harmful effects of freezing on cell membranes and reducing osmotic stress
Solution Approach 2:
The cryopreservation medium contains pre-added protective agents (cryoprotectants, albumin, disaccharides) that cushion cells against the harmful effects of freezing before intracellular freezing occurs, reducing membrane damage and solute concentration effects
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The described cryopreservation medium significantly improves the survivability and viability of immune cells, making it particularly suitable for cryopreserving adoptive cell therapies.
Implementation Method 1
cells subjected to freezing and thawing conditions are exposed to harsh conditions which in turn result in a generally low survivability and/or viability rate. Freezing is destructive to most living cells. Generally, as the extracellular medium freezes, cells attempt to maintain osmotic equilibrium across the membrane leading to intracellular water loss
Implementation Method 2
a cryopreservation medium described herein comprises a non-pyrogenic and isotonic crystalloid solution, a disaccharide, a cryoprotectant and an albumin
Data Source
AI summary
The present disclosure provides, among other things, a cryopreservation medium comprising a cryoprotectant, an albumin, a disaccharide and a non-pyrogenic and isotonic crystalloid solution. The disclosure also provides, among other things, a cryopreservation medium for cryopreserving immune cells, the medium comprising: human serum albumin (HSA), sodium chloride, sodium gluconate, sodium acetate trihydrate, potassium chloride, magnesium chloride, dimethyl sulfoxide (DMSO), and a trehalose. The present disclosure also provides, a method of cryopreserving immune cells, transporting and subsequently administering such immune cells to a patient in need thereof.