In Vitro Diagnostic Kit for Immune Cell Activity Analysis
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Solution Overview
Problem
Current in-vitro diagnostics for transplants, autoimmune diseases, tumor diseases, and vaccinations face challenges in accurately assessing immune cell activity due to inadequate consideration of complex feedback mechanisms, leading to unreliable results and time-consuming processes that can impair cell activity.
Innovation Solution
A diagnostic kit comprising a blood collection container, a nutrient medium, and activators, inhibitors, and modulators that allow immune cells to be cultured in whole blood, enabling analysis in their natural environment and maximizing standardization, thus improving the accuracy and reliability of results.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If immune cells are investigated separately from other blood components, then morphological and histological analyses can be performed, but functional analysis reliability deteriorates due to loss of complex feedback mechanisms
Solution Approach 1:
The patent combines immune cells with other blood components (platelets, erythrocytes, plasma) to perform functional analyses in whole blood or plasma whole blood, preserving the complex feedback mechanisms and messenger substance interactions that occur in vivo, thereby maintaining high functional analysis reliability
2Loss of information
If functional tests are performed on immune cells, then informative value increases, but time consumption and complexity increase due to selective culture requirements
Solution Approach 1:
The patent performs functional tests directly on whole blood or plasma whole blood without requiring selective immune cell culture, thereby obtaining high informative value about immune cell function while avoiding the time-consuming and complex culture procedures
Solution Approach 2:
The patent uses in vitro activation of immune cells in whole blood with various stimulants (LPS, PMA, ionomycin, etc.) to pre-establish functional states before analysis, enabling direct measurement of functional responses without lengthy culture periods
3Ease of operation
If immune cells are stored and transported for analysis, then analysis can be performed remotely, but cell activity impairment increases leading to distorted results
Solution Approach 1:
The patent analyzes immune cell function directly in whole blood or plasma whole blood samples without requiring isolation and storage of immune cells, thereby maintaining cell activity and reliability while enabling remote analysis through standard sample collection and transport procedures
Solution Approach 2:
The patent allows whole blood or plasma whole blood samples to maintain their native composition and physiological interactions during storage and transport, with immune cells functioning autonomously within the blood matrix, preserving their natural activity states for reliable analysis
Data Source
AI summary
The invention relates to the use in vitro of a kitfor diagnostics, more particularly therapy-accompanying diagnostics, relating to a transplantand/orfor diagnostics, more particularly therapy-accompanying diagnostics, relating to an autoimmune diseaseand/orfor diagnostics, more particularly therapy-accompanying diagnostics, relating to a tumor diseaseand/orfor diagnostics, more particularly therapy-accompanying diagnostics, relating to a vaccination,wherein the kit has the following components:a blood collection container, a blood collection set or blood collection system including a blood collection container,a nutrient medium for blood, andat least one activator for activating blood cells, more particularly immune cells, and/or at least one inhibitor for inhibiting blood cells, more particularly immune cells, and/or at least one modulator for modulating blood cells, more particularly immune cells.The invention further relates to the use in vitro of a method for diagnostics in transplants and/or autoimmune diseases and/or tumor diseases and/or vaccinations, to a messenger-substance-containing supernatant for use in vitro in diagnostics in transplants and/or autoimmune diseases and/or tumor diseases and/or vaccinations, and to a combination for use in diagnostics in transplants and/or autoimmune diseases and/or tumor diseases and/or vaccinations.