Immunity Detecting Kit for Cytokine Assessment of Herbal Extracts

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Solution Overview

Problem

Current methods lack an effective way to assess the impact of Chinese herbal medicines and nutritional products on individual immune responses, making it difficult to determine whether they enhance or suppress immune activity, and there is no standardized approach to formulate treatment strategies based on immune status.

Innovation Solution

An immunity detecting kit comprising an immune-stimulant agent (phorbol 12-myristate 13-acetate and lipopolysaccharide) and a combination of Chinese herb extracts or nutritional products, which are used to treat immune cells to assess cytokine expression, determining whether the combination has enhancing or suppressing effects on immune activity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional Chinese medicine studies use inconsistent experimental conditions and drug treatment times, then various Chinese herbal medicines can be tested for immune effects, but it becomes impossible to clearly understand the effects on immune response of blood cells of various individuals and assess immune status

Engineering Contradiction:

Improvetesting capability of various Chinese herbal medicinesVSAvoidassessment accuracy of immune status

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent standardizes key experimental parameters including treatment time (24 hours), cell concentration (1×10^6 cells/mL), and cytokine detection thresholds to enable consistent and comparable immune activity assessments across different Chinese herbal medicines and individual samples

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent implements a feedback mechanism by detecting cytokine levels (TNF-α, IL-6, IL-1β) as indicators of immune response, using these measurements to assess individual immune status and guide personalized treatment strategy formulation

Inventive Principle:

Principle #23Feedback

2Ease of operation

If no effective detection method is available, then Chinese herbal medicines and nutritional products can be freely used, but it is impossible to detect the impact on the immune system and assess immune activity

Engineering Contradiction:

Improveavailability of Chinese herbal medicines and nutritional productsVSAvoiddetected impact on immune system

Core Design Contradiction:

Ease of operationVSReliability

Solution Approach 1:

The patent uses cytokines (TNF-α, IL-6, IL-1β) as intermediary markers to detect and quantify the impact of Chinese herbal medicines and nutritional products on the immune system, translating complex immune responses into measurable indicators for reliable assessment

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces subjective clinical assessment with objective laboratory detection methods, using ELISA or other quantitative assays to measure cytokine levels and determine immune activity with precise numerical data

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If standardized detection is not implemented, then treatment strategies can be formulated based on general knowledge, but personalized treatment strategies based on individual immune status cannot be developed

Engineering Contradiction:

Improveformulation of treatment strategiesVSAvoidpersonalization of treatment strategy

Core Design Contradiction:

ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent applies local quality by tailoring treatment recommendations to each individual's specific immune status, using detected cytokine levels to determine whether an individual has hyperactive or hypoactive immune responses and prescribing accordingly

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The kit allows for the identification of Chinese herbal extracts or nutritional products with immunity-enhancing or -suppressing activities, enabling personalized treatment strategies by evaluating immune responses and improving immune function in individuals.

Implementation Method 1

promote the macrophages and the dendritic cells to secrete TNF-α, IFN-γ, IL-2 or other cytokines to enhance the immune response

Methodology Applied

Scientific EffectCytokine secretion:

Implementation Method 2

suppress the secretion of the inflammation-related cytokines such as TNF-α, IL-6, IL-1B, etc., so as to suppress the immune response

Methodology Applied

Scientific EffectAnti-inflammatory effect:

Data Source

PatentUS20250093333A1Immunity detecting kit and using method thereof

Publication Date: 2025.03.20 CHINA MEDICAL UNIVERSITY(TW)

AI summary

An immunity detecting kit includes an immune-stimulant agent and a Chinese herb combination or a nutrient combination. The immune-stimulant agent includes a phorbol 12-myristate 13-acetate (PMA) and a lipopolysaccharide (LPS). The Chinese herb combination includes at least one Chinese herb extract. The nutrient combination includes at least one nutritional product. A using method of immunity detecting kit is also provided.