Immunoadsorbent for Mycotoxin Purification and LC-MS Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for detecting mycotoxins like fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin are inefficient, prone to false positives and negatives, and require hazardous solvents, limiting their sensitivity and safety in agricultural and food product analysis.
Innovation Solution
Development of an immunoadsorbent and composite affinity column using monoclonal antibodies coupled to a solid phase carrier, specifically designed for purifying and detecting these mycotoxins, which allows for specific absorption and elution using chromatographic grade methanol, followed by high-performance liquid chromatography-mass spectrometry for precise quantification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional detection methods are used for mycotoxins, then detection can be performed, but sensitivity is low and false positives/negatives occur
Solution Approach 1:
The patent segments the detection process into two distinct stages: purification using immunoaffinity columns and detection using LC-MS/MS. This segmentation allows each stage to be optimized independently, with the purification stage removing interfering substances and the detection stage providing high sensitivity and accuracy, thereby resolving the contradiction between detection sensitivity and reliability.
Solution Approach 2:
The patent introduces an immunoaffinity purification column as an intermediary between the sample and the detection system. This intermediary selectively binds mycotoxins while removing interfering substances, providing a clean sample to the detection system. This intermediary step enhances both detection sensitivity by concentrating analytes and reliability by eliminating false positives/negatives from matrix interference.
2Object-affected harmful factors
If traditional detection methods are used, then detection can be performed, but hazardous solvents are required
Solution Approach 1:
The patent replaces traditional solvent-based extraction and cleanup methods with immunoaffinity-based purification. Instead of using hazardous solvents for extraction and cleanup, the system uses antibody-antigen specific binding followed by elution with minimal solvent, significantly reducing the use of hazardous chemicals while maintaining effective mycotoxin detection.
Solution Approach 2:
The patent changes the chemical parameters of the purification process by using aqueous buffers and minimal organic solvents for elution, replacing the traditional approach that requires large volumes of hazardous organic solvents. This parameter change reduces safety hazards while maintaining purification effectiveness.
3Productivity
If current detection methods are used, then analysis can be performed, but detection efficiency is low
Solution Approach 1:
The patent performs preliminary purification and concentration of mycotoxins using immunoaffinity columns before detection. This preliminary action removes interfering substances and concentrates analytes, reducing the time required for sample preparation and improving detection efficiency by providing a clean, concentrated sample ready for rapid LC-MS/MS analysis.
Solution Approach 2:
The patent merges the purification and concentration steps into a single immunoaffinity column operation, eliminating the need for separate cleanup and concentration procedures. This merging of functions reduces overall analysis time while improving detection efficiency by providing a clean, concentrated sample in one step.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The affinity column provides a stable, economical, quick, and safe method for purifying and detecting these toxins, reducing mutual interference and enabling accurate quantification without the need for hazardous solvents, thus enhancing the detection efficiency and safety in agricultural and food product analysis.
Implementation Method 1
an anti-fumonisin B1 monoclonal antibody, an anti-anguidin monoclonal antibody, an anti-T-2 toxin monoclonal antibody, an anti-zearalenone monoclonal antibody, and an anti-vomitoxin monoclonal antibody which are coupled to the solid phase carrier
Implementation Method 2
specifically designed for purifying and detecting these mycotoxins, which allows for specific absorption and elution using chromatographic grade methanol
Data Source
AI summary
An immunoadsorbent and a composite affinity column for purifying fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin. The immunoadsorbent includes a solid phase carrier, and a fumonisin B1 monoclonal antibody, an anguidin monoclonal antibody, a T-2 toxin monoclonal antibody, a zearalenone monoclonal antibody and a vomitoxin monoclonal antibody which are coupled to the solid phase carrier, the anguidin monoclonal antibody is a monoclonal antibody secreted by a hybridoma cell strain DAS5G11E7 having an accession number of CCTCCNO:C201881. The affinity column can be used for high performance liquid chromatography-mass spectrometry detection of the fumonisin B1, the anguidin, the T-2 toxin, the zearalenone and the vomitoxin, and has stable performance. Furthermore, an economical, quick, precise and safe detection method is established of the basis of the affinity column, and can be used for purifying and detecting samples of the five toxins without mutual interference and influence.