Immunoassay Binding Strength Detection via Displacement Kinetics
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Solution Overview
Problem
Existing methods for detecting analytes and determining their binding strength are often hindered by complex matrices like serum, leading to non-reproducible results and increased costs due to the need for additional avidity determination tests.
Innovation Solution
A procedure that involves immobilizing target molecules and reference analytes on a fixed surface, using detection fluorescence markers to measure binding, and then dissociating the analytes to determine their avidity, allowing for simultaneous detection and characterization of analytes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If existing methods are used for detecting analytes and determining binding strength separately, then detection accuracy is maintained, but analysis time increases and material consumption increases
Solution Approach 1:
The patent combines analyte detection and avidity determination into a single integrated assay. The method uses a displacement step where a second analyte competes with the first analyte for binding to the target molecule, allowing both detection and binding strength characterization to occur simultaneously in one experiment rather than requiring separate tests.
Solution Approach 2:
The assay system is designed to perform multiple functions: it detects the presence of analytes, quantifies their concentration, and determines their binding strength (avidity) all within the same experimental setup. The displacement-based methodology enables the system to extract multiple parameters from a single assay, making it a multi-functional analytical tool.
2Device complexity
If separate tests are performed for detection and avidity determination, then measurement reliability is maintained, but device complexity and cost increase
Solution Approach 1:
The patent integrates two separate analytical processes (detection and avidity determination) into a unified displacement assay. By performing both measurements in the same system with the same reagents and under the same conditions, the method reduces device complexity and eliminates the need for multiple separate instruments or assay setups.
Solution Approach 2:
The assay performs detection first, and only for samples that test positive does it proceed to the displacement step for avidity determination. This preliminary action approach ensures that the more complex displacement measurements are only performed when necessary, maintaining reliability while reducing overall complexity for negative samples.
3Loss of substance
If complex matrices like serum are used in separate detection and avidity tests, then comprehensive analysis is achieved, but result reproducibility decreases
Solution Approach 1:
The patent uses the same sample material (e.g., serum) for both detection and avidity determination within a single assay. This approach conserves material by not requiring separate samples for each test and ensures reproducibility by maintaining consistent sample conditions throughout the entire experimental process, eliminating variability introduced by sample handling between separate tests.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces analysis time, saves material, and enables the recording of dissociation kinetics, while also allowing for the use of existing automated systems for nucleic acid and immunoassay processing.
Implementation Method 1
using detection fluorescence markers to measure binding
Data Source
Figure 1~2
Figure 3~4B
AI summary
The present invention relates to a method for detecting and determining the binding strength of at least one analyte in a sample, based on the principle of an immunoassay. The method enables the detection and subsequent characterization of the binding properties of analytes in a single test. Furthermore, a kit for carrying out the method according to the invention is provided.