Immunoassay Device for Blood Type Antigen-Specific Immunoglobulin Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for blood type antigen-specific immunoglobulin detection and collection are limited by the complexity and multi-step processes involved, which can hinder efficient detection and utilization in transfusions and organ transplants.
Innovation Solution
An immunoassay device and method utilizing a substrate with immobilized binding antibodies and a switching peptide with a fluorescent label, which allows for one-step detection of blood type antigen-specific immunoglobulin by quantifying fluorescence released from the binding antibody.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multi-step processes are used for immunoglobulin detection and collection, then detection accuracy and collection efficiency can be improved, but process complexity and time consumption increase
Solution Approach 1:
The patent combines multiple detection and collection steps into a single integrated immunoassay device. The device merges antibody immobilization, antigen-antibody reaction, and immunoglobulin collection functions into one unified system, eliminating the need for separate multi-step procedures while maintaining detection accuracy through the coordinated interaction of binding antibodies, switching peptides, and fluorescent labels
Solution Approach 2:
The immunoassay device performs multiple functions simultaneously: it detects blood type antigen-specific immunoglobulins, collects the immunoglobulins on magnetic beads, and provides visual feedback through fluorescence signals. This multi-functional design allows a single device to replace multiple separate instruments and procedures
2Reliability
If multi-step processes are used for immunoglobulin detection and collection, then detection reliability can be improved, but time consumption increases
Solution Approach 1:
The patent employs preliminary action by pre-immobilizing binding antibodies on magnetic beads and pre-positioning switching peptides with fluorescent labels near the antibodies. This pre-preparation ensures that when the sample is introduced, the detection reaction can proceed immediately without delays for setup or preparation, maintaining reliability through pre-optimized conditions
Solution Approach 2:
The detection process maintains continuous useful action through the sustained binding interaction between antibodies and antigens, followed by continuous fluorescence signal generation. The magnetic beads remain in contact with the sample throughout the detection period, ensuring uninterrupted binding and signal production without requiring repeated additions or adjustments
3Productivity
If simplified one-step process is used, then process complexity and time consumption are reduced, but detection capability and collection efficiency may be compromised
Solution Approach 1:
The patent uses switching peptides as intermediaries between the immobilized binding antibodies and the fluorescent labels. These peptides facilitate the transfer of immunoglobulins from the antibody binding sites to the collection phase while maintaining the fluorescent signal, enabling both detection and collection to occur through a single coordinated mechanism rather than requiring separate complex procedures
Solution Approach 2:
The detection capability in the simplified one-step process is maintained through fluorescence color changes. When immunoglobulins bind to the antibody-peptide complex, the fluorescent label emits light at specific wavelengths, providing clear visual and measurable signals that confirm successful detection and binding events
4Object-affected harmful factors
If complex multi-step procedures are used for blood type matching, then immune response reduction can be optimized, but ease of operation decreases
Solution Approach 1:
The immunoassay device performs self-service by automatically completing the detection and collection functions through the inherent binding properties of the immobilized antibodies and switching peptides. When sample and reagents are introduced, the system autonomously carries out antigen-antibody reactions, immunoglobulin transfer to magnetic beads, and fluorescence signal generation without requiring complex manual operations or multiple intervention steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The proposed solution enables efficient, one-step detection and collection of blood type antigen-specific immunoglobulin, facilitating improved compatibility in blood transfusions and organ transplants by reducing immune response.
Implementation Method 1
a fluorescent label binding to the peptide compound
Implementation Method 2
a fluorescence-quenching substance positioned adjacent to the fluorescent label and quenching fluorescence from the fluorescent label
Implementation Method 3
the binding antibody includes one selected from the group consisting of a CDR3 including an amino acid sequence of SEQ ID NO 1, a CDR3 including an amino acid sequence of SEQ ID NO 2, and a CDR3 including an amino acid sequence of SEQ ID NO 3, and thus specifically binds to the blood type antigen-specific immunoglobulin
Data Source
AI summary
An immunoassay device for detecting blood type antigen-specific immunoglobulin, an immunoassay method for detecting blood type antigen-specific immunoglobulin, and a method for collecting blood type antigen-specific immunoglobulin are disclosed. The immunoassay device for detecting blood type antigen-specific immunoglobulin includes a substrate having a reaction space defined therein capable of receiving therein a detection target sample solution; a binding antibody positioned in the reaction space and immobilized on the substrate; a switching peptide having a peptide compound reversibly binding to a Fab region of the binding antibody and a fluorescent label binding to the peptide compound; and a fluorescence-quenching substance positioned adjacent to the fluorescent label and quenching fluorescence from the fluorescent label.