Immunoassay Reagent Storage Stability via Dummy Carrier Segmentation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing immunoassay reagents with insoluble carriers face issues of storage stability due to dissociation of immobilized substances, affecting measurement accuracy.
Innovation Solution
An immunoassay reagent comprising a combination of insoluble carriers bound and unbound to the reactive substance, using a binding pair like biotin and biotin-binding protein, enhances storage stability by allowing rebinding of dissociated substances.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a substance is immobilized at an insoluble carrier in an immunoassay reagent, then the reagent can be used for immune reaction detection, but the substance may dissociate from the carrier during storage, causing measurement values to fluctuate
Solution Approach 1:
The patent divides the insoluble carrier into two distinct populations: carriers with immobilized substance and dummy carriers without substance. This segmentation allows the dummy carriers to specifically absorb dissociated substance without interfering with the immune reaction, thereby resolving the contradiction between storage stability and measurement precision
Solution Approach 2:
The dummy carriers act as an intermediary component that mediates the dissociation issue. By providing additional binding sites that do not participate in the specific immune reaction, they capture dissociated substance and prevent it from affecting measurement accuracy, thus protecting the reliability of the assay
2Stability of the object's composition
If dummy particles are added to suppress dissociation, then storage stability improves, but the reagent composition becomes more complex
Solution Approach 1:
The dummy carriers are designed to be homogeneous in size, shape, and surface properties with the functional carriers, differing only in the absence of immobilized substance. This homogeneity simplifies the overall reagent system while maintaining the stability benefit, as the carriers behave similarly in the assay environment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Maintains favorable measurement accuracy by ensuring a surplus of binding sites for the reactive substance, reducing free substance in the sample, and stabilizing the reagent during storage.
Implementation Method 1
the substance and the insoluble carrier are bound to each other via a binding pair that consists of a pair of substances having a function to bind to each other
Implementation Method 2
the coexistence of the insoluble-carrier particulate reagent and the dummy particles causes a shift in a balance of dissociation/adsorption toward the adsorption-side
Data Source
AI summary
An immunoassay reagent, including: a substance that exhibits an immune reaction; and an insoluble carrier having a function to bind to the substance, the insoluble carrier including an insoluble carrier that is bound to the substance and an insoluble carrier that is not bound to the substance.