Immunoassay Non-Specific Reaction Suppression With Lambda-Kappa Antibodies
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Solution Overview
Problem
Existing immunoassay methods face challenges in suppressing non-specific reactions caused by heterophil antibodies and rheumatoid factors, which current non-specific reaction suppressing agents are unable to fully address, leading to measurement errors.
Innovation Solution
Incorporating anti-immunoglobulin L chain lambda and kappa monoclonal antibodies into the immunoassay process to suppress non-specific reactions, particularly in homogeneous methods like latex turbidimetric immunoassay, by performing immunoreactions in the presence of these antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional non-specific reaction suppressing agents (anti-human IgM, IgG, IgA polyclonal antibodies) are used, then some non-specific reactions are suppressed, but measurement errors persist due to unsuppressed non-specific reactions
Solution Approach 1:
The invention changes the specificity parameter of the suppressing agent from class-specific (IgM, IgG, IgA) to subtype-specific (L chain lambda and kappa), enabling more precise and comprehensive suppression of non-specific reactions while maintaining measurement accuracy
Solution Approach 2:
The invention segments the immunoglobulin structure into heavy chains and light chains, and further segments light chains into lambda and kappa subtypes. This segmentation allows the suppressing agent to target specific light chain types, providing more granular and effective suppression of non-specific reactions
2Reliability
If anti-human IgM polyclonal antibody is used as suppressing agent, then non-specific reactions are suppressed, but preparation is restricted to same animal type as measurement antibody
Solution Approach 1:
The anti-L chain lambda and anti-L chain kappa monoclonal antibodies serve as universal suppressing agents that can be used with any measurement antibody type (mouse, rabbit, goat, etc.), eliminating the restriction that the suppressing agent must be from the same animal type as the measurement antibody
Solution Approach 2:
The invention uses L chain antibodies as an intermediary that bridges different animal species. Since L chains are a universal component of all immunoglobulins regardless of species, the anti-L chain antibodies can mediate suppression across different animal types, enabling versatile application
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively suppresses non-specific reactions that conventional agents cannot, enabling accurate immunoassay measurements by reducing interference from non-specific factors.
Implementation Method 1
an immunoassay method which is an immunological measuring method of a target substance to be measured in a sample, the immunoassay method comprising performing an immunoreaction in a presence of an anti-immunoglobulin L chain lambda monoclonal antibody and an anti-immunoglobulin L chain kappa monoclonal antibody, in a presence of the anti-immunoglobulin L chain lambda monoclonal antibody, or in a presence of the anti-immunoglobulin L chain kappa monoclonal antibody
Data Source
AI summary
An immunoassay method that is an immunologically measuring method of a target substance to be measured in a sample, the immunoassay method including performing an immunoreaction in a presence of an anti-immunoglobulin L chain lambda monoclonal antibody and an anti-immunoglobulin L chain kappa monoclonal antibody, in a presence of the anti-immunoglobulin L chain lambda monoclonal antibody, or in a presence of the anti-immunoglobulin L chain kappa monoclonal antibody. This immunoassay method enables suppression of a non-specific reaction that could not be resolved by non-specific reaction suppressing agents in the related art.