Immunoassay Absorbance Monitoring for Prozone and Auto-Dilution
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Solution Overview
Problem
Existing immunological measurement methods face challenges in accurately measuring components with wide concentration ranges due to the prozone phenomenon, requiring complex and time-consuming dilution processes to bring samples within measurable ranges.
Innovation Solution
An analysis device and method that utilize absorbance measurements during the reaction course to detect the prozone phenomenon and automatically determine an optimal dilution rate, allowing for rapid and accurate concentration prediction and measurement without additional dilution steps.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional immunological measurement methods are used for samples with wide concentration ranges, then measurement accuracy deteriorates due to the prozone phenomenon, but adding surfactants or designing specific antibodies does not sufficiently suppress the phenomenon and increases device complexity
Solution Approach 1:
The system performs preliminary absorbance measurements during the reaction course to detect prozone phenomenon before final quantification. By measuring absorbance at multiple time points during the reaction, the system can identify prozone conditions early and trigger appropriate handling procedures, preventing inaccurate measurements without requiring complex preprocessing steps
Solution Approach 2:
The system implements feedback control by continuously monitoring absorbance changes during the reaction and comparing them against expected patterns. When prozone phenomenon is detected through abnormal absorbance profiles, the system automatically adjusts measurement parameters or triggers dilution protocols, creating a closed-loop control system that maintains measurement accuracy without manual intervention
2Measurement precision
If dilution operations are performed to bring high concentration samples within measurement range, then measurement accuracy improves, but measurement time increases due to repetitive dilution and remeasurement operations
Solution Approach 1:
The system performs preliminary absorbance measurements during the reaction course to detect prozone phenomenon before final quantification. By measuring absorbance at multiple time points during the reaction, the system can identify prozone conditions early and trigger appropriate handling procedures, preventing inaccurate measurements without requiring complex preprocessing steps
Solution Approach 2:
The system implements feedback control by continuously monitoring absorbance changes during the reaction and comparing them against expected patterns. When prozone phenomenon is detected through abnormal absorbance profiles, the system automatically adjusts measurement parameters or triggers dilution protocols, creating a closed-loop control system that maintains measurement accuracy without manual intervention
3Measurement precision
If manual dilution operations are conducted to achieve accurate measurement of high concentration samples, then measurement accuracy improves, but operational complexity and risk of error increase
Solution Approach 1:
The system performs self-diagnosis by automatically detecting prozone phenomenon through absorbance profile analysis. When prozone conditions are identified, the system autonomously initiates dilution operations and adjusts measurement parameters without requiring operator intervention, making the system as easy to operate as conventional methods while eliminating manual dilution errors
Solution Approach 2:
The system implements feedback control by continuously monitoring absorbance changes during the reaction and comparing them against expected patterns. When prozone phenomenon is detected through abnormal absorbance profiles, the system automatically adjusts measurement parameters or triggers dilution protocols, creating a closed-loop control system that maintains measurement accuracy without manual intervention
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate measurement of high concentration samples by predicting and adjusting dilution rates based on absorbance variations, simplifying the process and reducing the need for repetitive dilution operations.
Implementation Method 1
utilize an absorbance measurement result (alternatively called an absorbance variation or the like, and means absorbance variation, absorbance, difference in absorbance, rate of change in absorbance, and so on) in a reaction course
Data Source
AI summary
Provided are an analysis device, an analysis method, and the like that predict a concentration even for a sample having high concentration that can be outside a measurement range as it is, using an absorbance measurement result in a reaction course at the time of measurement of a test liquid containing an immunological reagent and the sample, and determine an optimum dilution rate to conduct measurement. As the present invention, for example, an analysis device having means (A) that detects a prozone during measurement of a sample, and means (B) that determines a high concentration region by automatically determining a dilution rate of the test liquid can be recited.