Immunoassay Reagent Immobilization for Higher RS Virus Sensitivity
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional immunoassay methods and reagents lack sufficient sensitivity, particularly in detecting RS virus, necessitating the development of a highly-sensitive immunoassay method and reagent.
Innovation Solution
The addition of disaccharides and sugar alcohols to a solution containing antigens, antibodies, or their binding fragments, enhances immobilization on carriers, thereby improving detection sensitivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional immunoassay methods are used, then the assay can be performed with standard reagents, but detection sensitivity is insufficient
Solution Approach 1:
The patent changes the chemical composition parameters of the immobilization solution by adding disaccharides (1-4% w/v) and sugar alcohols (0.5-4% w/v) to improve antigen/antibody immobilization efficiency on the carrier, thereby enhancing detection sensitivity by 1.5 times or more compared to conventional methods
Solution Approach 2:
The patent uses a composite immobilization solution containing both disaccharide and sugar alcohol components working together to achieve synergistic improvement in immobilization efficiency and detection sensitivity, creating a more effective reagent system
Data Source
AI summary
Provided are a highly-sensitive antibody and a test reagent using the antibody.A method for detecting an analyte according to an immunoassay method, using a carrier on which an antigen, an antibody or an antigen-binding fragment thereof is immobilized, whereinthe analyte is detected with increasing detection sensitivity by using a carrier to which an antigen, an antibody or an antigen-binding fragment thereof was immobilized by adding disaccharide and sugar alcohol to a solution comprising an antigen, an antibody or an antigen-binding fragment thereof.