Immunochip Quality Detection via Labeled Antibody Binding
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Solution Overview
Problem
The double-antibody sandwich method used for immunochip modification detection is labor-intensive and wasteful, with multiple steps increasing the risk of errors and reagent consumption, affecting the final detection effect.
Innovation Solution
A quality detection method involving a labeled antibody that reacts with modifying groups on the immunochip surface, allowing for the detection of residual labeled antibody content to assess the modification effect, which includes applying a detection solution, removing unreacted antibody, and identifying the immunochip quality based on fluorescence intensity for carboxyl-modified surfaces.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the double-antibody sandwich method is used to test the modification effect, then the detection can be performed, but it leads to high labor costs and serious reagent waste
Solution Approach 1:
The patent extracts and utilizes only the essential functional component (modifying groups) for detection, rather than requiring the complete double-antibody sandwich system. By detecting the modifying groups directly with a labeled antibody, the method eliminates unnecessary reagents and steps while maintaining detection capability.
Solution Approach 2:
Instead of detecting the target antigen through the traditional double-antibody sandwich method, the patent inverts the approach by detecting the modifying groups themselves. This inversion simplifies the detection system by focusing on the modification effect rather than requiring antigen-antibody complex formation.
2Measurement precision
If the double-antibody sandwich method is used, then the modification effect can be tested, but it has many operating steps which may cause errors
Solution Approach 1:
The patent extracts the core detection function by focusing solely on detecting modifying groups with a single labeled antibody, removing the complex multi-step double-antibody sandwich procedure. This extraction reduces operating steps from multiple stages to a single detection step.
Solution Approach 2:
The patent merges the detection of modification effect into a single step by using a labeled antibody that directly binds to modifying groups, combining what would otherwise require separate antigen binding and detection steps into one integrated operation.
3Reliability
If multiple operating steps are added, then the detection can be performed, but each additional step may cause errors affecting the final detection effect
Solution Approach 1:
The patent extracts the essential detection function by directly detecting modifying groups, removing intermediate steps that could introduce errors. This extraction maintains detection reliability by focusing on the fundamental modification effect rather than complex antigen-antibody interactions.
Solution Approach 2:
The patent inverts the traditional detection logic by detecting the modification groups themselves rather than using them as intermediaries for antigen detection. This inversion reduces the number of steps and potential error sources while maintaining or improving detection reliability.
4Measurement precision
If the double-antibody sandwich method is used, then the modification effect can be tested, but it leads to high labor costs
Solution Approach 1:
The patent extracts the core detection function by directly detecting modifying groups with a single labeled antibody, eliminating the labor-intensive double-antibody sandwich procedure. This extraction significantly reduces manual operations while maintaining detection accuracy.
Solution Approach 2:
The patent inverts the detection approach by targeting modifying groups directly rather than using the complex double-antibody sandwich method, thereby reducing labor requirements while preserving the ability to assess modification effects accurately.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies the detection process, reduces errors, conserves reagents, and enhances the reliability and efficiency of immunochip quality assessment by directly measuring the modifying groups' presence.
Implementation Method 1
the labeled antibody is capable of reacting with the modifying groups and bonding to the modifying groups
Implementation Method 2
the labeling substance is a fluorescent substance, and the labeled antibody is a fluorescent antibody
Data Source
AI summary
A quality detection method of an immunochip is disclosed. At least part of a surface of the immunochip is a modified surface with modifying groups. The method comprises: applying a detection solution containing a labeled antibody on the modified surface of the immunochip, the labeled antibody is an antibody connected with a labeling substance, and the labeled antibody is capable of reacting with the modifying groups and bonding to the modifying groups; removing the labeled antibody that has not reacted with the modifying groups, and detecting the content of residual labeled antibody on the modified surface; and identifying the quality of the immunochip according to the content of residual labeled antibody.