Lateral Migration Immunochromatographic Device for Quantitative Analyte Detection
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Solution Overview
Problem
Existing immunochromatographic devices are unable to provide quantitative determination of analytes and are prone to false results due to the 'hook' effect, especially at high concentrations, and require complex procedures and additional handling for sample dilution, making them unsuitable for domestic use.
Innovation Solution
A lateral migration immunochromatographic device with a capillary diffusion means that includes a deposition zone, a detection zone with immobilized analyte-specific binding reagents, and a third reagent, allowing simultaneous sandwich and competition tests without the need for sample dilution, enabling accurate detection of analytes across a wide concentration range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a sandwich test or competition test is used in conventional immunochromatographic devices, then the analyte can be detected, but quantitative determination is not possible and false results occur due to the 'hook' effect at high concentrations
Solution Approach 1:
The patent combines both sandwich test and competition test capabilities into a single immunochromatographic device with multiple detection zones. The first detection zone performs sandwich test for low concentration analytes, while the second detection zone performs competition test for high concentration analytes, enabling quantitative determination across a wide concentration range and eliminating the hook effect.
Solution Approach 2:
The detection zone is segmented into multiple independent detection zones, each optimized for specific concentration ranges. This segmentation allows different testing mechanisms to operate simultaneously on the same device, providing accurate quantitative measurement for both low and high analyte concentrations without interference.
2Measurement precision
If sample dilution is performed to overcome the hook' effect and expand the detection range, then measurement accuracy improves, but the procedure becomes complex and requires additional handling
Solution Approach 1:
The device automatically adapts its detection mechanism based on the analyte concentration in the undiluted sample. The dual-zone design with different test mechanisms allows the system to self-adjust for appropriate concentration ranges without requiring manual sample dilution or complex operational steps from the user.
3Measurement precision
If multiple separate devices are used for sandwich test and competition test, then each test can be optimized, but the device complexity and handling requirements increase
Solution Approach 1:
The patent integrates both sandwich test and competition test capabilities into a single immunochromatographic device with multiple detection zones. The first detection zone performs sandwich test for low concentration analytes, while the second detection zone performs competition test for high concentration analytes, enabling quantitative determination across a wide concentration range and eliminating the hook effect.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device allows for reliable and precise determination of analytes, avoiding false positives and negatives, and enables quantitative analysis without the need for sample dilution, making it suitable for domestic use and effective for analytes with significant hook effects.
Implementation Method 1
a capillary diffusion means (1), comprising in the direction of capillary diffusion (2)
Data Source
Figure 1~3B
Figure 4~7
AI summary
The invention relates to a device for determining an analyte in a liquid sample. The inventive device consists of: a capillary action means (1), involving lateral migration, defining a reference capillary action direction and comprising a liquid sample deposit area (3) and an analyte-detection area (5) which is disposed downstream of the deposit area; a first analyte-specific binding reagent (4) which is conjugated to a visible and/or measurable marker and which is free to migrate when wet by means of capillary action in the above-mentioned capillary action means (1) along the reference direction; and a second analyte-specific binding reagent (6) which is immobilised in the detection area. The invention is characterised in that the detection area (5) comprises the analyte (7) or an analogue of the analyte, which is immobilised and disposed at a distance from the second specific binding reagent (6).