Immunochromatographic Device Nitrous Acid Storage Stability
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Solution Overview
Problem
Conventional immunochromatographic devices containing organic acid and nitrite suffer from storage stability issues due to diffusion and generation of nitrous acid, leading to decreased extraction efficiency and sensitivity during use.
Innovation Solution
The immunochromatographic device is designed with specific arrangements of nitrous acid compounds and organic acids or derivatives, where these components are positioned upstream from the labeling substance-containing part, preventing contact and diffusion during storage, thereby inhibiting nitrous acid generation until use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If nitrous acid compound and organic acid are placed in contact or close proximity in the immunochromatographic device, then nitrous acid is generated efficiently during use for antigen extraction, but nitrous acid is generated prematurely during storage reducing stability
Solution Approach 1:
The device is divided into distinct functional zones: a first reagent application area containing the nitrous acid compound, a second reagent application area containing the organic acid, and a third reagent application area containing the antibody-conjugate. This spatial segmentation prevents premature contact between nitrous acid compound and organic acid during storage, while allowing their interaction during use through sample-driven mixing.
Solution Approach 2:
The patent utilizes the thickness direction (vertical dimension) of the test strip to separate the nitrous acid compound and organic acid into different layers or zones. This dimensional arrangement ensures that the two reagents do not substantially contact each other in the thickness direction during storage, preventing premature nitrous acid generation while maintaining extraction efficiency when activated.
2Ease of operation
If nitrous acid is generated during storage to simplify the extraction process, then the extraction step is simplified, but the detection sensitivity and extraction efficiency decrease over time
Solution Approach 1:
The nitrous acid compound and organic acid are pre-positioned in their respective application areas on the test strip during manufacturing, but their reactive action is delayed until use. The sample solution serves as the trigger that brings these reagents into contact, ensuring that nitrous acid is generated at the optimal moment for antigen extraction, thereby maintaining both operational simplicity and detection sensitivity.
Solution Approach 2:
The sample solution acts as an intermediary that mediates the interaction between the nitrous acid compound and organic acid. Instead of directly mixing the reagents during storage, the sample solution is introduced to bring these reagents into contact in the first reagent application area, enabling controlled nitrous acid generation only when needed for extraction.
3Ease of operation
If the nitrous acid compound and organic acid are mixed in advance, then the extraction process is simplified, but mistakes in mixing may occur leading to incorrect diagnosis
Solution Approach 1:
The test strip is designed to automatically perform the mixing function through capillary action and sample-driven transport. The sample solution automatically brings the nitrous acid compound and organic acid into contact in the first reagent application area, eliminating the need for manual mixing operations. This self-service mechanism ensures consistent, mistake-free reagent interaction while maintaining operational simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This arrangement enhances storage stability and ensures efficient generation of nitrous acid during use, improving detection sensitivity and accuracy.
Implementation Method 1
nitrous acid generated through reaction of the nitrous acid compound and the organic acid or the organic acid derivative
Implementation Method 2
a chromatography medium member having a detection part
Implementation Method 3
an absorption member
Data Source
AI summary
The invention relates to an immunochromatographic device which contains a nitrous acid compound and an organic acid or an organic acid derivative and which is for detecting a detection target in an analyte wherein a sample droplet-receiving member, a labeling substance-holding member, a chromatography medium member and an absorption member are arranged in a manner that a sample develops in this order and wherein a part containing the nitrous acid compound and a part containing the organic acid or the like are at upstream positions from the labeling substance-containing part, and the part containing the nitrous acid compound and the part containing the organic acid or the like are not substantially in contact with each other in the thickness direction.