Immunochromatography Sequence for Sensitive Particulate Detection

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Conventional immunochromatography methods suffer from insufficient detection sensitivity due to the surface of particulate substances being covered by labeling antibodies, reducing reactivity with capture antibodies.

Innovation Solution

A method involving immunochromatography that includes contacting a sample with a first specific binding substance on a membrane to capture particulate substances, followed by labeling with a second specific binding substance, and detecting the labeled particles, where the first binding substance is immobilized on the membrane and the second is bound to a labeling substance, enhancing sensitivity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If the surface of particulate substance is covered by labeling antibody before development on membrane, then the particulate substance can be labeled, but the reactivity with capture antibody is reduced

Engineering Contradiction:

Improvedetection sensitivityVSAvoidreactivity with capture antibody

Core Design Contradiction:

Measurement precisionVSReliability

Solution Approach 1:

The invention applies preliminary action by first capturing the particulate substance with the capture antibody on the membrane before labeling with the labeling antibody. This sequence ensures that the particulate substance is securely bound to the membrane first, creating available binding sites for subsequent labeling without compromising the initial capture efficiency. The capture step is performed in advance, establishing a stable foundation for the labeling step that follows.

Inventive Principle:

Principle #10Preliminary action

2Productivity

If conventional immunochromatography method is used, then the detection can be performed rapidly, but the detection sensitivity is insufficient

Engineering Contradiction:

Improvedetection speedVSAvoiddetection sensitivity

Core Design Contradiction:

ProductivityVSMeasurement precision

Solution Approach 1:

The invention segments the immunochromatography process into two distinct sequential steps: (1) capture of particulate substance by capture antibody on the membrane, and (2) labeling of captured particulate substance by labeling antibody. This segmentation allows each step to be optimized independently - the capture step ensures high binding efficiency while the labeling step enhances detection sensitivity, thereby achieving both rapid detection and high sensitivity that cannot be achieved in a single conventional step.

Inventive Principle:

Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Immunochromatography achieves quick and highly sensitive detection of particulate substances by improving detection sensitivity through this sequential binding process.

Implementation Method 1

contacting on a membrane a sample containing a particulate substance with a first specific binding substance for a first substance to be bound to capture the particulate substance

Methodology Applied

Scientific EffectSpecific binding:

Implementation Method 2

contacting the captured particulate substance into contact with a second specific binding substance for a second substance to be bound to label the particulate substance

Methodology Applied

Scientific EffectSpecific binding:

Implementation Method 3

the first specific binding substance is immobilized on the membrane

Methodology Applied

Scientific EffectImmobilization: Adsorption

Data Source

PatentUS12590967B2Method for detecting particulate substance using immunochromatography, and kit for same

Publication Date: 2026.03.31 DAI NIPPON TORYO CO LTD

AI summary

The present invention relates to a method for detecting a particulate substance by immunochromatography, the particulate substance including, on its surface, a plurality of substances to be bound containing a first substance to be bound and a second substance to be bound which may be the same or different with respect to each other, wherein the method includes the steps of: (1) contacting on a membrane a sample containing the particulate substance with a first specific binding substance for the first substance to be bound to capture the particulate substance with the first specific binding substance; (2) contacting the captured particulate substance with a second specific binding substance for the second substance to be bound to label the particulate substance; and (3) detecting the labeled particulate substance, wherein the first specific binding substance is immobilized on the membrane, and the second specific binding substance is bound to a metal nanoparticle.