Immunochromatography Test Strip Suppressing White Bleaching
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Solution Overview
Problem
Immunochromatographic detection methods face challenges with the 'white bleaching phenomenon' and low detection sensitivity, particularly for influenza B virus, leading to inaccurate measurements and reduced reproducibility.
Innovation Solution
Incorporating methanol into the immunochromatographic test strip sample-supply portion suppresses the white bleaching phenomenon and enhances detection sensitivity for influenza B virus by using a sample-diluting solution containing 0.1 to 20% methanol, which is added to the sample-supply portion before detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If immunochromatography is used for detecting analytes, then the detection method is simple and rapid, but measurement waveform disturbance occurs due to white bleaching phenomenon leading to reduced measurement precision
Solution Approach 1:
A buffer solution is introduced as an intermediary substance between the sample and the membrane. This buffer solution contains components that prevent the white bleaching phenomenon from occurring on the membrane, thereby eliminating the measurement waveform disturbance while maintaining the simplicity of the immunochromatographic detection method
Solution Approach 2:
The chemical composition parameters of the buffer solution are specifically optimized to prevent white bleaching. By adjusting the buffer solution's pH, ionic strength, and component concentrations, the invention suppresses the harmful white bleaching phenomenon while preserving the rapid and simple nature of immunochromatographic detection
2Duration of action of stationary object
If immunochromatography is performed after long-term storage, then the test strip remains available for use, but white bleaching phenomenon occurs reducing reliability
Solution Approach 1:
The buffer solution is prepared in advance and contains pre-formulated components that actively prevent white bleaching phenomenon. This preliminary preparation ensures that even after long-term storage, when the test strip is finally used, the white bleaching phenomenon does not occur, maintaining measurement reliability throughout the storage period
Solution Approach 2:
The buffer solution acts as a protective cushioning agent that is already in place before storage begins. This pre-established protective environment prevents the white blaching phenomenon from developing during long-term storage, thereby preserving the reliability of the test strip throughout its storage life
3Speed
If conventional immunochromatography is used for influenza B virus detection, then the detection process is rapid, but detection sensitivity is low
Solution Approach 1:
The buffer solution serves as a mediator that enhances the interaction between the influenza B virus and the detecting antibody on the membrane. By optimizing the buffer's chemical environment, the invention improves viral antigen-antibody binding efficiency, thereby increasing detection sensitivity while maintaining the rapid detection capability
Solution Approach 2:
The buffer solution's parameters (pH, ionic composition, concentration) are specifically optimized for influenza B virus detection. These parameter adjustments enhance the immunoreaction efficiency between the virus and detecting reagents, achieving high detection sensitivity without compromising the rapidity of the immunochromatographic process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively suppresses the white bleaching phenomenon, enabling accurate measurements and significantly improves the detection sensitivity for influenza B virus, addressing the limitations of existing immunochromatographic techniques.
Implementation Method 1
the presence of methanol on the test strip suppresses the occurrence of the so-called white bleaching phenomenon of the test strip
Implementation Method 2
A detection method using an immunochromatographic test strip is known as a method of detecting an analyte (a substance to be detected) in a sample through an antigen-antibody reaction
Implementation Method 3
spreading an immune complex formed by an analyte and a labeled conjugate along with a mobile phase such as a buffer solution through a stationary phase that is an insoluble membrane support
Data Source
Figure 1~3
AI summary
The present invention provides a detection method using an immunochromatographic test strip that suppresses the occurrence of a so-called white bleaching phenomenon and that is accurate and excellent in sensitivity. The present invention provides an immunochromatographic detection method that uses an immunochromatographic test strip including (1) and (2) below and that comprises the steps of (A) and (B) below: (1) a conjugate pad having a sample-supply portion for supplying a sample possibly containing an analyte, and a conjugate portion on the downstream side of the sample-supply portion, the conjugate portion containing a conjugate in which an antibody to the analyte is immobilized on a label, (2) an insoluble membrane support having at least one detecting section on which an antibody to the analyte is immobilized, (A) a step of supplying methanol and the sample possibly containing the analyte to the sample-supply portion, and (B) a step of detecting the analyte as an immune reaction product on the insoluble membrane support.