ImmunoFOS Postbiotic Modulates Cytokine Release
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Solution Overview
Problem
There is a need for a postbiotic that can effectively modulate the immune system and protect mucosal barriers, particularly in newborns and premature neonates who are susceptible to infections due to their underdeveloped immune systems, and existing solutions do not adequately address the modulation of inflammatory responses and maintenance of epithelial barrier integrity.
Innovation Solution
A composition comprising a fermented supernatant derived from Lactobacillus casei or paracasei species, specifically ImmunoFOS, which is obtained through fermentation of fructo-oligosaccharides (FOS) by Lactobacillus paracasei strain CNCM I-5220, and is characterized by its ability to control immune responses by promoting the release of anti-inflammatory cytokines and maintaining epithelial barrier function.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a postbiotic is used to modulate the immune system and protect mucosal barriers, then immune response modulation and barrier protection are improved, but the complexity of obtaining and characterizing the postbiotic increases
Solution Approach 1:
The patent extracts and isolates specific postbiotic components (metabolites, peptides, proteins) from the fermentation supernatant through sequential purification steps including centrifugation, filtration, and chromatography. This extraction process enables the postbiotic to be separated from the complex microbial mixture, facilitating standardized production and characterization while maintaining the immunomodulatory and protective functions.
Solution Approach 2:
The patent employs multiple characterization techniques that analyze and modify parameters of the postbiotic such as molecular weight, concentration, purity, and biological activity. By systematically varying and measuring these parameters, the invention establishes quality control standards and ensures consistent immunomodulatory effects across different batches.
2Productivity
If Lactobacillus casei or paracasei species are used for fermentation, then postbiotic production is improved, but the difficulty of ensuring consistent strain performance increases
Solution Approach 1:
The patent specifies particular strain characteristics and genetic markers (such as 16S rRNA gene sequences) to identify and select Lactobacillus casei or paracasei strains with consistent postbiotic production capabilities. By focusing on specific local qualities of the strain (genetic composition, metabolic profile), the invention ensures reproducible performance across different production batches.
Solution Approach 2:
The patent implements quality control measures that monitor and evaluate postbiotic production parameters during fermentation. By providing feedback on strain performance, production yield, and product quality, the system enables real-time adjustments to maintain consistent strain performance and product characteristics.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
ImmunoFOS effectively reduces the release of pro-inflammatory cytokines like IL-12p40 and increases anti-inflammatory cytokine IL-10, thereby modulating immune responses and protecting mucosal barriers from pathogens, as demonstrated by its immunomodulatory and anti-inflammatory properties in both in vitro and in vivo studies.
Implementation Method 1
The invention refers to a composition comprising Lactobacillus casei or paracasei species postbiotic, preferably for the modulation of immune system and the protection of mucosal barriers... obtained through fermentation of fructo-oligosaccharides (FOS) by Lactobacillus paracasei strain CNCM I-5220
Data Source
AI summary
The present invention refers to a composition containing a) a fermented supernatant, or fractions thereof, of Lactobacillus casei or paracasei species, the species being characterized by having in their DNA genome at least one DNA sequence essentially identical to one of the sequence selected from the group consisting of: SEQ ID No 1 to 5, and b) proper carriers and/or diluents and/or excipients. The fermented supernatant is obtainable through a method characterized by the fermentation of the Lactobacillus casei or paracasei species in a minimum solution additioned with prebiotic fibers. The fermented supernatant does not have prebiotic fibers.