Immunomagnetic Biosubstance Separation Apparatus
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Solution Overview
Problem
Current biosubstance separation methods in liquid biopsy, such as liquid biopsy, face challenges including incomplete capture of target biosubstances leading to false negatives, residual impurities, and damage to biosubstances, with existing apparatuses limited to a single separation process.
Innovation Solution
A separating apparatus and method utilizing immunomagnetic beads, magnetic separation racks, and a controller to selectively execute positive or negative separation processes based on target biosubstance types, enabling precise isolation of biosubstances through automated processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If positive separation process is used to directly separate target biosubstances, then target biosubstances can be isolated, but most target biosubstances may fail to be captured resulting in false negatives
Solution Approach 1:
The patent introduces magnetic beads as intermediary carriers that bind to target biosubstances (such as circulating tumor cells) through antibodies. These magnetic beads serve as mediators between the target cells and the magnetic separation system, enabling more effective capture and separation of rare biosubstances from complex biological samples.
2Reliability
If negative separation process is used to remove non-target biosubstances, then target biosubstances are preserved, but residual solution contains many impurities
Solution Approach 1:
The patent implements a multi-stage separation process that segments the purification workflow into distinct steps: initial magnetic bead binding, first magnetic separation to remove majority of non-target cells, intermediate washing steps, and final elution. This segmented approach progressively removes impurities while preserving target biosubstances throughout the process.
3Device complexity
If existing separating apparatus uses single separation method, then device structure is simple, but cannot switch between positive and negative separation processes according to user requirements
Solution Approach 1:
The patent designs a magnetic separation apparatus with universal functionality that can perform both positive separation (capturing target cells) and negative separation (removing non-target cells) using the same core components. The system uses programmable control to switch between different separation modes, making the device adaptable to various user requirements without requiring separate specialized equipment for each separation type.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances the accuracy and reproducibility of biosubstance separation by allowing selection of appropriate processes, reducing manual steps and improving detection reliability.
Implementation Method 1
The magnetic separation racks are respectively disposed in the accommodating regions. Each of the magnetic separation racks is used to magnetically attract the immunomagnetic beads.
Implementation Method 2
Each of the magnetic separation racks is used to magnetically attract the immunomagnetic beads
Implementation Method 3
The magnetic bead reagent includes a plurality of immunomagnetic beads. The immunomagnetic beads are used for binding to the target biosubstances.
Data Source
AI summary
A separating apparatus of biosubstance and a separating method of the same are provided. The separating method includes: providing a controller to select a positive separation process or a negative separation process to execute according to types of target biosubstances; providing a pipette pump to inject a first magnetic bead reagent or a second magnetic bead reagent into a sample according to the selected separation process, such that first immunomagnetic beads of the first magnetic bead reagent or second immunomagnetic beads of the second magnetic bead reagent are used for binding to the target biosubstances; providing a magnetic separation rack to enrich the target biosubstances so as to separate the target biosubstances and non-target biosubstances; and providing the pipette pump to separate the target biosubstances and the non-target biosubstances.