Immunostaining Condition Calculator for Biological Sample Analysis
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Solution Overview
Problem
The detection accuracy of target molecules is influenced by reactivity and non-specific binding of binding molecules, making it challenging to set optimal staining conditions, which is a time-consuming process even for experienced users.
Innovation Solution
An information processing device and method that acquires signals from biological samples, calculates immunostaining conditions based on specific and non-specific signals, and outputs optimal conditions for reagent concentrations, including antibody clones, reaction times, and solution compositions, to enhance detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If binding molecules are used to detect target molecules, then detection capability is improved, but non-specific binding increases causing decreased detection accuracy
Solution Approach 1:
The patent applies parameter changes by systematically varying reagent concentrations, reaction times, and incubation conditions to identify optimal parameters that maximize specific binding while minimizing non-specific binding. The system automatically tests multiple parameter combinations and selects the set that achieves the best signal-to-noise ratio, thereby resolving the contradiction between detection capability and non-specific binding.
2Measurement precision
If staining conditions are manually set by user, then flexibility is maintained, but time consumption increases and optimal conditions may not be achieved
Solution Approach 1:
The patent implements preliminary action by pre-establishing a database of optimal staining conditions derived from extensive experimental data and by automatically determining morphology information before staining. This allows the system to quickly recommend or automatically apply optimal conditions without requiring time-consuming manual optimization, thereby reducing time loss while maintaining high detection accuracy.
Solution Approach 2:
The system incorporates feedback mechanisms by automatically evaluating the results of staining experiments and using this information to refine future condition selections. The morphology information and signal intensity data are fed back into the system to improve subsequent staining condition recommendations, creating a self-optimizing loop that reduces time consumption while improving accuracy.
3Measurement precision
If multiple reagent concentrations are tested, then optimal conditions can be found, but the complexity of the process increases
Solution Approach 1:
The patent applies self-service by implementing an automated system that independently performs the complex task of testing multiple reagent concentrations, analyzing results, and determining optimal conditions. The morphology determination unit and condition recommendation unit work autonomously to evaluate multiple concentrations and select the optimal one, thereby managing process complexity through automation rather than requiring manual intervention for each parameter.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach simplifies the setting of staining conditions, improves detection accuracy by minimizing non-specific binding, and reduces the time required for users to achieve optimal results.
Implementation Method 1
molecules such as antigen proteins are detected and/or analyzed using antibodies
Implementation Method 2
detection and analysis of molecules according to nucleic acid hybridization using a fluorescence-labeled nucleic acid probe
Implementation Method 3
Each fluorescent dye has unique properties, for example, a unique fluorescence spectrum and fluorescence intensity
Implementation Method 4
a data display method of displaying a fluorescence spectrum obtained by integrating or averaging detection data obtained by simultaneously detecting fluorescence emitted from microparticles
Data Source
AI summary
To provide a technique for supporting setting of staining conditions for a reagent containing binding molecules when target molecules are detected and/or analyzed.There is provided an information processing device including a signal acquisition unit that acquires a signal derived from a sample including a biological sample, a processing unit that calculates immunostaining conditions of a reagent for the sample including the biological sample based on the signal, and an output unit that outputs the immunostaining conditions, wherein the signal includes a signal derived from the reagent containing target molecules in the biological sample and binding molecules that are able to bind to the target molecules and/or a signal derived from non-target molecules and the reagent.