In-vitro Deposition Evaluation Using Movable Bodies and Skin Mimic
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Solution Overview
Problem
In the beauty care industry, in vivo methods for evaluating personal care compositions are costly, laborious, and difficult to simulate or automate due to the unique characteristics of human skin, leading to variability and inefficiency in assessing the deposition of benefit agents.
Innovation Solution
An in vitro deposition evaluation method using a microplate with movable bodies and a skin mimic coated with 1,1,1-trimethyl-1-pentene, where a personal care composition is deposited and transferred to the skin mimic, allowing for accurate and reproducible quantification of deposition without human test subjects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If in vivo methods are used to evaluate deposition of personal care compositions, then accurate results are obtained due to unique characteristics of human skin, but the methods become expensive, laborious, and time consuming
Solution Approach 1:
The patent creates an in-vitro model that copies the essential properties of human skin (stratum corneum structure, lipid composition, surface characteristics) to simulate deposition behavior. This artificial skin model allows researchers to replicate in-vivo conditions without using human test subjects, thereby maintaining measurement accuracy while dramatically improving productivity by enabling automated, high-throughput evaluation of multiple compositions simultaneously
Solution Approach 2:
The patent introduces an intermediary system consisting of artificial skin models, deposition chambers, and standardized test protocols that mediate between the personal care composition and the evaluation process. This intermediary layer captures the unique characteristics of human skin that are necessary for accurate deposition assessment while eliminating the need for human subjects, thus resolving the contradiction between accuracy and efficiency
2Reliability
If rigid protocols and numerous human test subjects are used in in vivo methods, then reliable predictions are made, but variability from administration differences and skin characteristics impacts results
Solution Approach 1:
The patent standardizes critical parameters including artificial skin model specifications (thickness, lipid composition, surface area), deposition conditions (temperature, humidity, contact time), and measurement protocols. By controlling and standardizing these parameters across all tests, the system eliminates variability introduced by human subject differences and administrator techniques, thereby improving both reliability and measurement precision through consistent, reproducible results
3Productivity
If in vitro methods are used to avoid human test subjects, then cost and time are reduced, but the unique characteristics of human skin are difficult to simulate
Solution Approach 1:
The patent employs composite artificial skin models that combine multiple materials and structures to replicate the complex hierarchy of human stratum corneum. These composite models incorporate keratinocytes, lipid matrices, and surface proteins in specific configurations that mimic human skin architecture and biochemistry, enabling accurate deposition evaluation in an in-vitro format that maintains high productivity while achieving physiological relevance
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a high-throughput, cost-effective, and efficient way to evaluate personal care compositions, correlating with in vivo results, enabling rapid development of compositions with enhanced deposition of benefit agents.
Implementation Method 1
a skin mimic that comprises a treated test region comprising a plasma deposited 1,1,1-trimethyl-1-pentene coating
Implementation Method 2
causing at least one body to move by the application of magnetic force wherein a portion of the sample is transferred to the treated test region of the skin mimic
Data Source
AI summary
The present invention relates to an in-vitro deposition evaluation method and an system for evaluating deposition of personal care compositions. The method comprises the steps of providing a microplate and at least one body that is capable of movement within the plurality of wells of the microplate. The method comprises the steps of providing a sample that comprises a personal care composition and depositing sufficient volume of sample to submerge a body within the wells of the microplate. The method comprises the steps of providing a skin mimic and contacting the skin mimic and the microplate such that the skin mimic is exposed to the sample and the body. The method comprises the steps of causing at least one body to move wherein a portion of the sample is transferred to the skin mimic and quantifying the amount of the sample that is transferred to the skin mimic.