Inbred Corn Line G07-NPID5459 for Stable Hybrid Seed Production

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Solution Overview

Problem

The challenge in corn breeding is to develop inbred lines that combine desirable traits such as high yield, disease resistance, and adaptability across different regions of the Corn Belt, while avoiding the limitations of traditional hybrid production methods that often result in segregating progeny and require extensive breeding efforts.

Innovation Solution

The development of the inbred corn line G07-NPID5459, which includes a method for producing hybrid seed and plants with specific traits like male sterility, herbicide resistance, and disease resistance, utilizing tissue culture and marker-assisted breeding to introduce targeted traits and reduce the need for backcrossing, thereby enhancing genetic stability and adaptability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional hybrid production methods are used, then hybrid seed can be produced, but the progeny become segregating and require extensive breeding efforts

Engineering Contradiction:
Improvegenetic stabilityVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by developing inbred lines through multiple generations of self-pollination and selection before hybrid production. The inbred lines are pre-sorted and pre-characterized for desired traits, so that when hybrids are produced, the genetic composition is already optimized, reducing the need for extensive post-production breeding efforts and ensuring genetic stability in the progeny.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by manipulating the genetic parameters of the inbred lines through controlled self-pollination and selection over multiple generations. By changing the genetic composition parameters to achieve homozygosity and desired trait expression, the patent creates stable inbred lines that produce uniform hybrid progeny, eliminating segregation issues.

Inventive Principle:
Principle #35Parameter changes

2Ease of manufacture

If CMS (cytoplasmic male sterility) is introduced into inbred lines, then hybrid seed production is simplified, but poor agronomic performance traits are introduced

Engineering Contradiction:
Improvehybrid seed productionVSAvoidagronomic performance
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent applies local quality by carefully selecting and maintaining specific genetic regions and traits within the inbred lines. By focusing on local genetic quality in specific chromosomal regions and selecting for desirable agronomic traits, the patent ensures that the inbred lines have both the necessary male sterility characteristics and good agronomic performance, avoiding the introduction of poor traits.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent utilizes feedback by implementing multi-year field testing and evaluation of the inbred lines and resulting hybrids. The performance data from these tests is fed back into the breeding program to select and refine the best lines, ensuring that only inbreds with both desired male sterility and good agronomic performance are used for hybrid production.

Inventive Principle:
Principle #23Feedback

3Manufacturing precision

If inbred lines are developed by selecting corn lines and selfing for several generations, then homozygous pure inbred lines are produced, but the process is time-consuming

Engineering Contradiction:
ImprovehomozygosityVSAvoidinbreeding generations
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent applies preliminary action by conducting multiple generations of self-pollination and selection before the actual hybrid production. This pre-processing of the inbred lines ensures that they are fully homozygous and stable, so that when hybrids are produced, the desired genetic precision is already achieved, reducing the need for additional time-consuming inbreeding steps.

Inventive Principle:
Principle #10Preliminary action

4Adaptability or versatility

If multiple traits are introduced into inbred lines, then hybrid performance is improved, but breeding complexity increases

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding program into distinct phases and modules: developing inbred lines with specific traits, testing them in controlled environments, evaluating hybrid performance, and selecting the best combinations. This segmentation allows complex multi-trait inbreds to be developed systematically, reducing the overall complexity by breaking down the breeding process into manageable steps.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS7763781B1Inbred corn line G07-NPID5459
Publication Date: 2010.07.27 SYNGENTA CROP PROTECITON AG

AI summary

Basically, this invention provides for an inbred corn line designated G07-NPID5459, methods for producing a corn plant by crossing plants of the inbred line G07-NPID5459 with plants of another corn plant. The invention relates to the various parts of inbred G07-NPID5459 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line G07-NPID5459 and plants produced by said methods.