Inclined Partition Cell Isolation Container
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Solution Overview
Problem
Current methods for isolating CD56-positive cells from skeletal muscle tissue are inefficient and vary in outcome due to manual shredding and enzymatic degradation, leading to inconsistent yields and viability of collected living cells.
Innovation Solution
A method involving a container with a partition that separates upper and lower spaces, allowing for controlled pressing of the tissue to loosen binding without damaging cells, combined with enzymatic treatment, to increase the number and purity of isolated stem cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual shredding and enzymatic degradation are used to isolate CD56-positive cells from skeletal muscle tissue, then the isolation process can be performed, but the yield and viability of collected living cells are inconsistent and relatively low
Solution Approach 1:
The container is divided into an upper space and a lower space by a partition. The partition includes an inclined portion that guides cells from the upper space to the lower space, enabling systematic separation and collection of isolated cells while maintaining consistent flow and preventing loss.
Solution Approach 2:
The partition acts as an intermediary structure between the tissue sample in the upper space and the collection area in the lower space. It facilitates controlled cell passage through the inclined portion while preventing direct mixing, ensuring consistent and reliable cell isolation.
2Productivity
If strong pressing is applied to crush tissue to loosen binding, then cell isolation efficiency improves, but cell damage and destruction increase
Solution Approach 1:
The partition provides localized structural support and guidance in critical areas where cells transition from the upper to lower space. The inclined portion is specifically designed to gently guide cells without applying damaging forces, while other areas of the container maintain their original properties for effective pressing.
3Device complexity
If the partition is horizontal to the bottom, then the structure is simple, but cells cannot be effectively separated and collected in the lower space
Solution Approach 1:
The partition includes an inclined portion that creates an asymmetric structure rather than a simple horizontal division. This asymmetry enables cells to naturally flow along the slope from the upper space to the lower space under gravity, significantly improving collection efficiency while adding minimal structural complexity.
Solution Approach 2:
The partition extends in multiple dimensions within the container, creating a three-dimensional separation structure. The inclined portion introduces a slope dimension that facilitates cell movement and separation, transforming a two-dimensional horizontal partition into an effective three-dimensional cell guidance system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach stabilizes the isolation process, significantly increasing the number and viability of collected living cells, particularly stem cells, while maintaining high purity, as demonstrated by comparative examples.
Implementation Method 1
a partition which separates an upper space and a lower space of the container main body... the partition configured to restrict the movement of the solution within the container
Implementation Method 2
an enzymatic degradation treatment that are performed by hand have been mainly performed for the isolation of the CD56-positive cells from the skeletal muscle tissue
Data Source
AI summary
A container is disclosed for isolating living cells. The container for crushing a sample by pressing includes a container main body having an opening part and a partition which separates an upper space and a lower space of the container main body, in which at least a part of the partition has a portion which is not horizontal with respect to the bottom of the container.