Inclined-Tip Cell Suction Pipette for Secure Embryo Cell Holding
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Solution Overview
Problem
Existing suction pipettes face challenges in securely holding cells without causing damage, particularly due to the need for strong suction that can dislodge cells and the risk of cells falling off the pipette tip, and difficulty in efficiently separating suctioned cells from the fertilized egg or embryo.
Innovation Solution
A cell suction holding pipette with a hollow body featuring an inclined surface on its leading end portion, designed to guide cells smoothly onto the pipette tip and ensure secure holding, and a cell suction collecting pipette with a smaller diameter leading end for efficient cell collection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cells are suctioned strongly to ensure secure holding, then cell holding reliability is improved, but cell damage increases
Solution Approach 1:
The suction force is localized to specific regions through the inclined surface design, creating areas of different suction intensity. The inclined surface directs cells toward the suction port while concentrating the holding force at the cell contact region, allowing secure holding without applying excessive suction force across the entire cell structure.
Solution Approach 2:
The inclined surface acts as an intermediary element between the suction port and the cell. It guides cells smoothly onto the suction port and distributes the suction force, preventing direct application of strong suction force that could damage the cell while still achieving secure holding.
2Ease of operation
If cells are discharged above the outer surface of the leading end portion, then cell transfer is facilitated, but cells may fall to the side of the pipette
Solution Approach 1:
The inclined surface provides a curved transition path that guides cells smoothly from the discharge location to the suction port. This curved geometry prevents cells from falling to the side by creating a continuous sloping path that directs cells inward toward the suction port rather than allowing them to drop outward.
Solution Approach 2:
The inclined surface adds a dimensional element to the cell transfer path, creating a sloped surface that guides cells in a controlled manner. This transforms the cell transfer from a simple vertical discharge to a controlled sliding motion along the inclined surface, preventing lateral falls while maintaining ease of operation.
3Stability of the object's composition
If a surrounding wall is added to hold cells laterally, then cell holding stability is improved, but cell damage from pressing increases
Solution Approach 1:
The cell holding function is segmented into multiple components: the suction port provides primary holding force, the inclined surface provides guidance and secondary support, and the surrounding wall provides lateral stability. This segmentation allows each component to perform its specific function without excessive force, preventing cell damage from pressing while maintaining stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates secure and damage-free cell suctioning and collection operations, reducing the risk of cell loss and damage during handling.
Implementation Method 1
the inside of the internal passage 3 is suctioned to generate negative pressure via the connection port by operating the injector. As a result, the operation target 1 such as a cell is suctioned and held by the suction port 2
Implementation Method 2
the discharged cell moves along the inclined surface portion 4 toward the leading end and downward as shown in FIG. 19
Data Source
Figure 1~3
Figure 4~6
Figure 7~9
AI summary
A cell suction holding pipette 1 includes a hollow body 2 and a lumen 3 having a leading end opening 31 and a rear end opening 32. The hollow body 2 includes a leading end portion 21, and the leading end portion 21 includes an inclined surface portion 4 formed on an outer surface of the leading end portion 21 and inclining toward a leading end side extension line of a central axis of the lumen 3.