Induced Tissue Regeneration Compositions Targeting COX7A1

Solution Overview

Problem

Current methods for tissue regeneration in mammals are inadequate, particularly in addressing trauma and degenerative diseases, as the molecular mechanisms for regeneration in animals like planaria and amphibians are not understood in humans, and existing treatments for transplant rejection and tissue regeneration are not effective.

Innovation Solution

The use of agents that inhibit COX7A1 expression, such as RNAi agents, antisense oligonucleotides, and inhibitory antibodies, to enhance or inhibit tissue regeneration by targeting specific genes and gene products, including PCDHB2, PCDHB17, COX7A1, and others, to promote or inhibit wound healing and tissue regeneration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If stem cell transplantation is used to treat tissue dysfunction, then tissue regeneration is improved, but transplant rejection occurs

Engineering Contradiction:

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies parameter changes by modifying the genetic composition of stem cells through hemizygosity in the MHC region. This genetic parameter modification allows stem cells to evade immune detection while maintaining their regenerative capacity, thereby resolving the contradiction between effective tissue regeneration and transplant rejection

Inventive Principle:

Principle #35Parameter changes

2Object-affected harmful factors

If parthenogenic stem cell lines are used to reduce HLA complexity, then transplant rejection is reduced, but antigen expression is completely eliminated

Engineering Contradiction:

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

Instead of completely eliminating antigen expression through parthenogenesis, the patent inverts the approach by creating hemizygous MHC regions that retain antigen expression at reduced levels. This inverted strategy maintains immune system engagement while reducing rejection risk, resolving the contradiction between minimizing rejection and preserving immune balance

Inventive Principle:

Principle #13The other way round (Inversion)

3Productivity

If COX7A1 expression is inhibited to enhance tissue regeneration, then wound healing is improved, but off-target effects may occur

Engineering Contradiction:

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent uses RNA interference technology as an intermediary mechanism to specifically inhibit COX7A1 expression. This intermediary approach allows precise targeting of the COX7A1 gene through sequence-specific RNA molecules, enhancing wound healing while minimizing off-target effects by ensuring high specificity of the interference mechanism

Inventive Principle:

Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enhances or inhibits tissue regeneration by modulating the expression of key genes, facilitating scarless wound healing and tissue repair in mammals, providing therapeutic strategies for trauma and degenerative diseases.

Implementation Method 1

The use of agents that inhibit COX7A1 expression, such as RNAi agents

Methodology Applied

Scientific EffectRNA interference:

Implementation Method 2

antisense oligonucleotides, and inhibitory antibodies, to enhance or inhibit tissue regeneration by targeting specific genes

Methodology Applied

Scientific EffectAntisense oligonucleotide binding:

Implementation Method 3

The use of agents that inhibit COX7A1 expression, such as RNAi agents, antisense oligonucleotides, and inhibitory antibodies

Methodology Applied

Scientific EffectAntibody-antigen binding:

Data Source

PatentEP3888640B1Compositions and methods for induced tissue regeneration in mammalian species

Publication Date: 2025.09.17

Figure 1A~1B
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AI summary

Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. Said methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration.