Injectable EMD-Cell Composition for Gingival Soft Tissue Regeneration

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Solution Overview

Problem

Current surgical procedures for soft tissue reconstruction in the craniomaxillofacial complex, particularly for patients with thin gingival biotypes, are invasive and carry a high risk of secondary infection and inflammatory complications, with limited success in achieving ideal functional and aesthetic outcomes.

Innovation Solution

A minimally-invasive composition comprising purified enamel matrix derivative proteins and human fibroblasts or periodontal ligament cells, optionally with additional ECM proteins, administered via injection or hydrodissection, to promote soft tissue growth and healing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If surgical procedures are used for soft tissue reconstruction, then tissue availability and closure of defects are improved, but trauma and risk of secondary infection increase

Engineering Contradiction:
Improveclosure of defectsVSAvoidtrauma and infection risk
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses a composition comprising enamel matrix derivative (EMD) proteins and human fibroblasts or periodontal ligament cells as an intermediary substance that can be injected directly into the defect site. This eliminates the need for surgical harvesting of tissue flaps while providing the necessary cells and growth factors to promote tissue regeneration and closure of defects, thereby reducing trauma and infection risk associated with traditional surgical procedures

Inventive Principle:
Principle #24Intermediary (Mediator)

2Quantity of substance

If local flap harvests are used to increase tissue availability, then closure of defects is improved, but functional and aesthetic goals are not ideally met

Engineering Contradiction:
Improvetissue availabilityVSAvoidfunctional and aesthetic outcomes
Core Design Contradiction:
Quantity of substanceVSManufacturing precision

Solution Approach 1:

The patent applies local quality by delivering a concentrated composition of EMD proteins and specific cell types (fibroblasts or periodontal ligament cells) directly to the defect site. This localized delivery ensures that the regenerative process occurs precisely where needed, with the EMD proteins providing site-specific signals for tissue formation that match the functional and aesthetic requirements of the oral cavity, rather than relying on general tissue flaps from distant sites

Inventive Principle:
Principle #3Local quality

3Reliability

If traditional surgical methods are used for gingival reconstruction, then tissue replacement is achieved, but treatment time and recovery period are extended

Engineering Contradiction:
Improvetissue replacementVSAvoidtreatment time and recovery
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by pre-culturing human fibroblasts or periodontal ligament cells and combining them with EMD proteins in a ready-to-inject composition. This pre-prepared formulation contains all necessary components for tissue regeneration in optimal concentrations, eliminating the need for lengthy surgical procedures and extended recovery periods. The composition can be injected directly into the defect site and begins promoting tissue regeneration immediately, significantly reducing overall treatment time while maintaining reliable tissue replacement outcomes

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition enhances soft tissue regeneration with reduced trauma and infection risk, improving tissue quality and treatment outcomes for thin gingival biotypes, including gingival thickening, recession treatment, and peri-implantitis prevention.

Implementation Method 1

The composition enhances soft tissue regeneration with reduced trauma and infection risk

Methodology Applied
Scientific EffectTissue regeneration:

Data Source

PatentEP4656174A1Injectable compositions comprising enamel matrix derivative (EMD) proteins and human soft tissue cells
Publication Date: 2025.12.03 INSTITUT STRAUMANN AG
  • EP4656174A1 patent drawingFigure 1
  • EP4656174A1 patent drawingFigure 2
  • EP4656174A1 patent drawingFigure 3

AI summary

The present invention relates to a pharmaceutical, dental and/or cosmetic composition comprising a cell culture population of human fibroblasts (hPFB) and/or human periodontal ligament cells (hPDL), and purified and/or isolated enamel matrix derivative (EMD) proteins, which have a molecular weight between 1-160kDa and its use in treating patients suffering from a gingival deficiency and/or disorder, in particular such patients suffering from a gingival deficiency and/or disorder selected from the group consisting of gingival thin biotype, gingival recession and enlargement and gingival papilla regeneration. Preferably, the composition of the invention is administered via injection into the soft tissue in the oral cavity and/or the craniomaxillofacial complex (CMF) of the patient.