Injectable Crosslinked Hyaluronic Acid–DNA Composition for Enzyme Resistance

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Solution Overview

Problem

Conventional hyaluronic acid-based fillers lack functional composites with skin regeneration effects and are prone to enzyme degradation due to the use of non-crosslinked hyaluronic acid.

Innovation Solution

A hyaluronic acid composition is developed by mixing crosslinked hyaluronic acid derivatives with DNA fractions, specifically polynucleotide (PN) or polydeoxyribonucleotide (PDRN), at a weight ratio of 7.0 to 9.5:0.5 to 3.0, to enhance viscoelasticity and resistance to hyaluronidase.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of operation

If non-crosslinked hyaluronic acid is used to prepare filler products, then good feeling during operation is achieved, but the product is easily degraded by enzyme reaction

Engineering Contradiction:
Improvegood feeling during operationVSAvoidenzyme resistance
Core Design Contradiction:
Ease of operationVSReliability

Solution Approach 1:

The patent creates a composite filler product combining crosslinked hyaluronic acid derivatives with DNA fractions. The crosslinked HA provides enzyme resistance while the DNA fractions contribute to skin regeneration effects, achieving both reliability and functional benefits

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The patent modifies the degree of crosslinking of hyaluronic acid to specific ranges (0.1-200% as weight ratio of crosslinking agent to HA monomer) to optimize both operational properties and enzyme resistance, balancing ease of operation with stability

Inventive Principle:
Principle #35Parameter changes

2Productivity

If conventional hyaluronic acid fillers are used, then filling and cosmetic purposes are achieved, but skin regeneration effect is lacking

Engineering Contradiction:
Improvecosmetic efficacyVSAvoidskin regeneration function
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent merges crosslinked hyaluronic acid derivatives with DNA fractions in a specific weight ratio (7.0 to 9.5:0.5 to 3.0) to create a composite filler that provides both conventional cosmetic filling effects and additional skin regeneration functions through DNA-mediated extracellular matrix formation

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The composite filler product achieves multiple functions simultaneously: tissue filling, wrinkle correction, and skin regeneration through DNA fraction stimulation of extracellular matrix formation, making it applicable for various cosmetic and therapeutic purposes

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Reliability

If crosslinked hyaluronic acid derivatives are used, then enzyme resistance is improved, but viscoelasticity may be compromised

Engineering Contradiction:
Improveenzyme resistanceVSAvoidviscoelasticity
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent optimizes the degree of crosslinking within specific ranges (0.1-200% as weight ratio) to maintain adequate viscoelasticity while achieving sufficient enzyme resistance, balancing both properties through parameter control

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The composite formulation with DNA fractions compensates for any viscoelasticity changes introduced by crosslinking, maintaining operational properties while achieving enhanced enzyme resistance

Inventive Principle:
Principle #40Composite materials

Data Source

PatentEP3381438B2Composition for injection of hyaluronic acid, containing hyaluronic acid derivative and DNA fraction, and use thereof
Publication Date: 2025.07.16 BMI KOREA CO LTD
  • EP3381438B2 patent drawingFigure 1a
  • EP3381438B2 patent drawingFigure 1b
  • EP3381438B2 patent drawingFigure 1c

AI summary

The present invention refers to the injectable hyaluronic acid composition which is a crosslinked viscoelastic hyaluronic acid gel comprising DNA fractions. More particularly, it refers to the injectable hyaluronic acid composition comprising a hyaluronic acid composition crosslinked in the basic conditions with the degree of crosslinking between 0.1 and 200%, which is mixed with the DNA fractions of 0.1 to 50 wt%. The DNA fractions are selected, for example, from polynucleotide (PN) and polydeoxyribonucleotide (PDRN). The composition used for cosmetic purposes or therapeutic purposes, have improved viscoelastic rheological properties and enzyme resistance.