Novel Insecticidal Proteins for Broad-Spectrum Pest Control
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Solution Overview
Problem
Current genetically engineered crops that produce pesticidal proteins from Bacillus strains provide limited resistance to insect pests and are susceptible to resistance development, necessitating the identification of alternative biological control agents with broader insecticidal activity.
Innovation Solution
Development of novel nucleic acid molecules encoding insecticidal polypeptides such as IPD078-1, IPD078-2, IPD084, IPD086-1, IPD086-2, IPD087, and IPD089, which can be used to transform bacteria and plants to confer pesticidal activity, including against Lepidopteran, Coleopteran, nematode, fungi, and Dipteran pests, and methods for detecting these polypeptides and their nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If genetically engineered crops produce pesticidal proteins from Bacillus strains, then insect resistance is provided, but the range of insecticidal activity is narrow and insects can develop resistance
Solution Approach 1:
The patent applies universality by engineering crops to produce multiple different pesticidal proteins from various Bacillus strains simultaneously. This multi-functionality approach allows a single crop variety to provide protection against multiple insect orders (Lepidoptera, Coleoptera, Diptera, Hemiptera) rather than relying on a single protein with limited scope, thereby resolving the contradiction between providing reliable insect resistance and expanding the range of insecticidal activity.
Solution Approach 2:
The patent employs composite materials by combining multiple pesticidal proteins with different modes of action and target specificities within the same transgenic crop. This composite strategy creates a synergistic effect where the combination of proteins provides broader spectrum control and reduces the likelihood of resistance development compared to single-protein approaches, addressing both the reliability and versatility requirements.
2Productivity
If genetically engineered crops produce pesticidal proteins, then pest control effectiveness is improved, but resistance development in insects occurs
Solution Approach 1:
The patent applies segmentation by dividing the pest control function into multiple distinct pesticidal proteins, each targeting different insect physiological pathways or species groups. This segmentation ensures that when insects develop resistance to one protein, they remain susceptible to others, thereby maintaining pest control effectiveness while reducing overall susceptibility to resistance development.
Solution Approach 2:
The patent utilizes parameter changes by incorporating pesticidal proteins with diverse modes of action, target sites, and spectral activities into the transgenic crops. By varying these critical parameters across multiple proteins, the system achieves robust pest control while minimizing the risk that insects can adapt to a single mode of action, thus resolving the contradiction between effectiveness and resistance susceptibility.
Data Source
AI summary
Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.