Insecticidal Protein Compositions for Bt Resistance Management
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Solution Overview
Problem
Current pesticidal proteins derived from Bacillus thuringiensis are facing resistance issues in target pests, necessitating the development of novel insecticidal proteins with new modes of action to effectively control plant pests.
Innovation Solution
Transformation of plants with nucleic acid molecules encoding polypeptides having at least 80% sequence identity to SEQ ID NOs: 1-184, which express pesticidal proteins with at least 95% sequence identity, providing effective control against plant pathogens and pests.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current pesticidal proteins derived from Bacillus thuringiensis are used, then crop protection is achieved, but pest resistance develops
Solution Approach 1:
The patent applies parameter changes by modifying amino acid sequences of pesticidal proteins to create variants with altered properties. Specifically, the patent describes modifying conserved and variable regions of Cry proteins to enhance pesticidal activity while reducing resistance development in target pests like fall armyworm and European corn borer.
Solution Approach 2:
The patent employs composite materials by combining modified pesticidal protein variants with other pesticidal proteins or compounds to create compositions with synergistic effects. This stacking approach addresses resistance by using multiple modes of action simultaneously, making it harder for pests to develop resistance.
2Adaptability or versatility
If novel pesticidal proteins with new modes of action are developed, then pest resistance is reduced, but protein engineering complexity increases
Solution Approach 1:
The patent applies segmentation by dividing the pesticidal protein into functional domains: conserved regions that maintain core pesticidal activity and variable regions that can be modified to create new modes of action. This modular approach allows systematic engineering of novel proteins while maintaining fundamental functionality.
Solution Approach 2:
The patent applies local quality by making specific localized modifications to amino acid sequences at particular positions within the protein structure. Rather than random mutations, the patent targets specific conserved and variable regions to achieve desired properties while minimizing unintended effects on overall protein function.
Data Source
AI summary
Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.