Inspection Chip Side Surface Reaction Site
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional inspection chips with reaction sites on the bottom surface face issues with liquid removal and detection accuracy due to liquid residue and surface meniscus effects, and asymmetric designs for side surface reaction sites lead to unstable stirring and inefficient reagent supply.
Innovation Solution
An inspection chip with a side surface reaction site and a well design that allows for stable circular movement and efficient stirring, featuring a curved bottom surface and a side wall member with a metal film and capturing film for enhanced surface plasmon resonance, enabling improved detection accuracy and reagent distribution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If the reaction site is arranged on the bottom surface of the well, then the structure is simple and easy to manufacture, but liquid residue remains and detection accuracy is lowered
Solution Approach 1:
The reaction site is moved from the bottom surface (horizontal plane) to the side surface (vertical plane) of the well. This dimensional change allows the liquid delivery tool tip to be positioned above the reaction site without contacting it, enabling complete liquid removal while maintaining the reaction site's accessibility for biochemical reactions and fluorescence detection.
2Productivity
If the liquid delivery tool tip is pressed against the bottom surface to remove liquid, then liquid removal is sufficient, but the metal film or reaction site is broken
Solution Approach 1:
By relocating the reaction site to the side surface, the liquid delivery tool tip can be positioned vertically above the reaction site during liquid removal. This spatial separation in the vertical dimension allows the tool to contact only the liquid and bottom surface, eliminating the risk of damaging the metal film or reaction site while ensuring complete liquid removal.
3Productivity
If the reaction site is arranged on the side surface, then liquid removal is improved, but the chip becomes asymmetric leading to unstable stirring
Solution Approach 1:
The reaction site is intentionally designed on the side surface rather than the bottom, creating an asymmetric configuration. This asymmetric design enables the liquid delivery tool to remove liquid completely without contacting the reaction site, as the tip can be positioned vertically above the side-surface reaction site, achieving both complete liquid removal and protection of the reaction site integrity.
4Device complexity
If the detection unit is arranged above the well, then the structure is simple, but detection accuracy is affected by meniscus and air bubbles
Solution Approach 1:
The reaction site is repositioned from the bottom surface to the side surface of the well. This dimensional relocation allows the detection unit to detect fluorescence from the side surface reaction site, avoiding interference from the liquid meniscus and air bubbles that are present at the top surface, thereby improving detection accuracy while maintaining a relatively simple detection system structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration enhances detection accuracy and ensures stable, efficient stirring of reagents, preventing liquid residue and surface interference, while maintaining chip stability during rotation.
Implementation Method 1
the fluorescent substance is excited by an electric field enhanced by surface plasmon resonance (SPR) to emit fluorescence
Implementation Method 2
the fluorescent substance is excited by an electric field enhanced by surface plasmon resonance (SPR) to emit fluorescence
Data Source
Figure 1
Figure 2
Figure 3
AI summary
This inspection chip for stirring a liquid via the circular movement of a bottom surface end has a well body for storing the liquid, and a lateral wall member positioned on the lateral surface of the well body. The bottom surface end has a bottom surface structure which: contacts a rotation member for circularly moving the bottom surface end; and is located at a position offset toward the lateral wall member from the center line of the well body.