Integrated Bio-Analysis System with Built-In PCR Thermal Cycling
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Solution Overview
Problem
Current DNA analysis procedures are time-consuming and expensive due to the complexity of equipment and the need for manual interventions during sample preparation and separation, which can be simplified by integrating sample preparation processes within the analysis system.
Innovation Solution
A bio-analysis system with a built-in sample preparation device, such as a PCR-based system, that includes a thermal cycling unit for automated DNA amplification, allowing for direct sample preparation and analysis within the same instrument, reducing user intervention and increasing throughput.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate dedicated devices are used for sample preparation and CE analysis, then each device can be optimized for its specific function, but the overall system complexity increases and manual intervention is required
Solution Approach 1:
The patent combines the sample preparation device and capillary electrophoresis analysis device into a single integrated system. The sample preparation module includes thermal cycling capabilities for PCR and interfaces directly with the CE separation module, eliminating the need for separate dedicated devices and manual transfer operations while maintaining functional optimization for both sample preparation and analysis.
Solution Approach 2:
The integrated system performs multiple functions within a single device: it can conduct thermal cycling for PCR amplification, separate DNA fragments via capillary electrophoresis, and detect fluorescent signals. This multi-functionality reduces system complexity by consolidating what would otherwise require separate specialized devices.
2Adaptability or versatility
If manual sample preparation and transfer procedures are used, then flexibility in handling different samples is maintained, but time consumption increases and throughput decreases
Solution Approach 1:
The integrated system enables automated self-service operation where the sample preparation module directly interfaces with the CE analysis module. Samples are automatically transferred from the thermal cycling chamber to the capillary electrophoresis separation channel without manual intervention, maintaining flexibility while dramatically increasing throughput and reducing analysis time.
Solution Approach 2:
The system performs preliminary sample preparation actions (thermal cycling for PCR amplification) automatically within the integrated device before analysis. This preliminary automation eliminates subsequent manual steps and enables continuous high-throughput operation while maintaining adaptability for different sample types.
3Reliability
If multiple separate devices are used for DNA analysis workflow, then each device can be specialized, but the cost of equipment and operation increases
Solution Approach 1:
The patent merges the sample preparation device and CE analysis device into a single integrated instrument, reducing the total number of devices required. This consolidation maintains specialization quality for both PCR and electrophoresis functions while significantly reducing equipment purchase costs and operational expenses associated with multiple separate devices.
Solution Approach 2:
The integrated device achieves multi-functionality by incorporating thermal cycling capabilities, capillary electrophoresis separation, and fluorescent detection in one system. This universality eliminates the need for multiple specialized devices, reducing overall equipment cost while maintaining the specialization quality needed for reliable DNA analysis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The integrated system reduces the time and cost of DNA analysis by automating sample preparation and analysis, enabling high-throughput, low-cost, and sensitive genetic analysis, with the ability to detect biological agents and pathogens efficiently.
Implementation Method 1
a thermal cycling unit for automated DNA amplification
Implementation Method 2
Electrophoresis techniques could be used to separate fragments of DNA for genotyping applications
Implementation Method 3
detecting the fluorescence emissions induced by the incident light
Data Source
AI summary
An integrated bio-analysis system incorporates built-in sample preparation capabilities. In one aspect of the present invention, a bio-analysis instrument is provided with a built-in sample preparation device based on PCR (or thermal cycling block/module). In one embodiment of the present invention, a peltier unit in the sample preparation device provides thermal cycling of samples supported in a multi-well tray. In another aspect of the present invention, a CE instrument is provided with a built-in sample preparation capability, which may comprise a sample preparation (bio-molecular reaction) device based on thermal cycler type. In another aspect of the present invention, a PCR device is provided with a built-in analysis device, such as a CE device, for verifying the results of the PCR (bio-molecular reaction) process.