Transgenic Betta With Chromosomally Integrated Fluorescent Transgenes
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Solution Overview
Problem
Progress in developing transgenic betta fish that express fluorescent proteins for enhanced ornamental fish display has been limited, and there is a need for novel and aesthetically pleasing colors in the ornamental fish industry.
Innovation Solution
The development of transgenic betta fish with specific integration events, such as Green Betta 1, Orange Betta 1, Red Betta 1, Purple Betta 1, and Blue Betta 1, using chromosomally integrated transgenes, which are bred and distributed to the ornamental fish market, and can be produced through methods involving cryopreserved testes and in vitro fertilization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If transgenic technology is used to introduce fluorescent proteins into betta fish, then novel and enhanced ornamental colors can be achieved, but progress has been limited and development has been slow
Solution Approach 1:
The patent applies preliminary action by using cryopreserved testes from transgenic betta fish as a starting material. This allows the transgenic traits to be pre-established in a stable, preservable form before being introduced to new fish through in vitro fertilization, thereby accelerating the development process compared to traditional methods.
Solution Approach 2:
The patent replaces traditional mechanical breeding methods with in vitro fertilization technology. This substitution enables precise control over the introduction of transgenic traits and significantly speeds up the development of new fluorescent color varieties in betta fish.
2Adaptability or versatility
If traditional breeding methods are used to create color variations, then stable coloration can be achieved, but novel fluorescent colors cannot be introduced
Solution Approach 1:
The patent replaces traditional mechanical breeding with in vitro fertilization and chromosomally integrated transgenes. This substitution enables the reliable introduction of novel fluorescent colors through genetic engineering while ensuring stable inheritance through chromosomal integration, overcoming the limitations of both traditional breeding and earlier transgenic methods.
Solution Approach 2:
The patent applies parameter changes by modifying the genetic material at the molecular level through transgene integration. This changes the fundamental parameter of color production from natural pigment synthesis to fluorescent protein expression, enabling novel colors while maintaining stability through chromosomal integration.
3Productivity
If early transgenic methods were used to introduce foreign genes, then some color changes could be achieved, but the process was complex and progress was limited
Solution Approach 1:
The patent replaces complex traditional transgenic methods with in vitro fertilization combined with chromosomally integrated transgenes. This substitution simplifies the overall process by establishing stable genetic integration early in development, thereby increasing productivity while reducing the complexity of subsequent breeding operations.
Solution Approach 2:
The patent applies preliminary action by establishing chromosomally integrated transgenes in the founder generation using cryopreserved testes. This preliminary establishment of stable genetic integration simplifies all subsequent breeding operations and significantly increases the productivity of generating new transgenic varieties.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These transgenic betta fish exhibit stable and visually distinct fluorescent colors, providing improved ornamental value and potential scientific research tools, such as tracing cell lineage and marking cells in genetic mosaic experiments.
Implementation Method 1
transgenic betta fish that express fluorescent proteins for enhanced ornamental fish display
Data Source
AI summary
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.