Integrated Motor Platform for Lab-on-Disc Real-Time Cell Monitoring
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Solution Overview
Problem
Existing Lab-on-disc (LoD) systems face challenges in implementing active, real-time sensing and detection while spinning, and they lack the capability for long-term cultivation of bacteria or mammalian cells.
Innovation Solution
A mobile/portable centrifugal microfluidic device with a rotatable platform that integrates electrical motors for mechanical energy, enabling real-time monitoring and long-term cell culture through controlled fluid flow and nutrient supply.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If an external spindle motor is used to drive the LoD system, then the platform can rotate, but the system becomes bulky and difficult to control
Solution Approach 1:
The motor is integrated directly onto the rotatable platform, merging the drive mechanism with the platform itself. This eliminates the need for external motors and complex transmission systems, reducing overall device complexity while maintaining rotation capability
Solution Approach 2:
The integrated motor serves multiple functions: it provides rotational drive, can generate electrical energy through regenerative braking or dedicated generators, and reduces the need for separate control systems. This multi-functionality addresses both rotation requirements and energy needs within a single component
2Measurement precision
If traditional strobe photography setup is used for imaging, then real-time monitoring is possible, but the setup becomes bulky and requires dedicated equipment
Solution Approach 1:
The imaging system is integrated directly onto the rotatable platform, combining cameras, lights, and processing electronics with the rotating sample holder. This eliminates the need for separate external imaging equipment and complex synchronization systems
Solution Approach 2:
The patent replaces traditional mechanical strobe photography systems with electronic imaging and processing. Digital cameras and LED lights integrated on the platform capture images continuously or at specific rotational positions, eliminating the need for mechanical shutters and external flash systems
3Measurement precision
If LoD system is used for real-time sensing, then detection capability is improved, but implementation becomes difficult while spinning
Solution Approach 1:
Sensing electrodes and detection electronics are integrated directly onto the rotatable platform, merging the detection system with the rotating sample holder. This allows sensing elements to move with the sample, maintaining detection capability throughout rotation without requiring complex external synchronization
Solution Approach 2:
The integrated sensing system performs multiple detection functions (electrochemical, optical, electrical) simultaneously while rotating, eliminating the need for separate detection systems for each measurement type and simplifying operation
4Measurement precision
If bulky electrochemical detection systems are used, then detection accuracy is maintained, but portability is reduced
Solution Approach 1:
Electrochemical detection electrodes and electronics are integrated directly onto the rotatable platform, combining detection functionality with the rotating sample holder. This eliminates the need for separate external potentiostats and detection equipment, significantly reducing system weight
Solution Approach 2:
The detection system is segmented into miniaturized components that can be distributed across the rotating platform surface. Multiple small electrodes and sensing elements replace a single large external detection system, reducing overall weight while maintaining detection accuracy through parallel measurement capability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device allows for efficient real-time monitoring and long-term cultivation of cells, mimicking in vivo conditions, while reducing the need for bulky equipment and trained personnel.
Implementation Method 1
a rotatable platform on top of the base configured to rotate with respect to an axis perpendicular to the base... configured to rotate with the rotatable platform such that the fluid sample is centrifuged during rotation of the rotatable platform
Data Source
AI summary
A rotatable platform is provided for use in Lab-on-disc (LoD) applications. A LoD microfluidic device has the rotatable platform and is suitable for analysis of a fluid sample. Real-time monitoring of cells is also provided, using a centrifugal microfluidic platform. A mobile LoD device is provided, which can be used in remote destinations and for point of care applications. A method for monitoring microorganisms under the constant supply of nutrients includes the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear/centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation of the platform by means of imaging, electrochemical and/or electrical measurements.