Interference Filter Angle Correction for Spectral Accuracy
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Solution Overview
Problem
High throughput imaging systems face challenges in accurately correcting transmission spectra due to angular dispersion of light, which causes spectral shifts as light strikes the emission filter at varying angles of incidence, leading to inaccurate sample imaging.
Innovation Solution
The system employs a method to correct the transmission spectrum intensity based on the angle of incidence by generating a correction image using convolutions of the emission spectrum and transmission spectra at different angles, normalizing these values to account for spectral shifting.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If high throughput imaging is performed with large field of view, then imaging productivity is improved, but angular dispersion of light causes spectral shifts that worsen measurement precision
Solution Approach 1:
The system dynamically adjusts the angle of incidence parameter by tilting the emission filter relative to the optical axis. This allows the filter to be optimally positioned for different field of view regions, compensating for angular dispersion and maintaining spectral accuracy across the entire imaging area while preserving high throughput capability
Solution Approach 2:
The emission filter is made movable rather than fixed, allowing real-time adjustment of its angular position. This dynamic repositioning enables the system to adapt to varying illumination angles across the large field of view, eliminating spectral shifts without reducing imaging productivity
2Area of stationary object
If emission filter is positioned to capture large field of view, then area of stationary object is improved, but angle of incidence deviation causes transmission spectrum intensity variations that worsen manufacturing precision
Solution Approach 1:
The system changes the angular parameter of the emission filter by tilting it to different angles corresponding to different field of view positions. This ensures that the filter operates at optimal incidence angles across the entire large field of view, maintaining consistent transmission spectrum intensity and accuracy without compromising the expanded imaging area
3Measurement precision
If manual correction of transmission spectrum is performed, then measurement precision may be improved, but time consumption increases that reduces productivity
Solution Approach 1:
The system pre-calculates and stores the relationship between angle of incidence and transmission spectrum intensity variations during the manufacturing or setup phase. This preliminary characterization allows the system to apply corrections automatically and instantaneously during imaging operations, achieving high spectral accuracy without time-consuming manual correction processes
Solution Approach 2:
The system replaces manual correction procedures with an automated computational algorithm that uses the pre-established angle-intensity relationship. This substitution eliminates human intervention in the correction process, maintaining high measurement precision while dramatically reducing the time required for spectral correction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach results in a more accurate image representation by normalizing the intensity variations across the emission filter's field of view, effectively correcting for spectral shifts caused by different angles of incidence, thereby enhancing the accuracy of in vivo imaging.
Implementation Method 1
spectral shifts in the transmission spectrum from the emission filter... Based on a spectral response of the emission filter as a function of the angles of incidence
Data Source
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AI summary
Aspects of the present disclosure provide systems, methods, devices, and computer-readable media for interference filter correction based on angle of incidence. In some examples, a sample emits an emission spectrum that is filtered by an emission filter to provide a transmission spectrum. The emission spectrum illuminates the emission filter at multiple angles of incidence. The angles of incidence result in a spectral shifting of the transmission spectrum. Based on this spectral shifting, the intensity of the transmission spectrum is corrected. An image corresponding to the corrected intensity of the transmission spectrum may be generated.