Ion Spectrometry Cycles for Metabolite ID and Quantitation
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Solution Overview
Problem
Current bioanalytical methods using ion spectrometry, such as mass analysis, face limitations in efficiently quantitating analyte compounds and assigning chemical identities, particularly when dealing with low molecular weight metabolites, as they often require comprehensive data sets that are challenging to acquire and process effectively.
Innovation Solution
A method involving two ion spectrometric measurement cycles is employed, where a pooled sample is analyzed for comprehensive physical-chemical properties to generate a first spectral dataset, and individual samples are analyzed for a reduced set of properties to generate a second dataset, with the data from both used to create distinct tables for quantitation and chemical assignment, leveraging common descriptors for accurate translation between qualitative and quantitative data.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If comprehensive data sets are acquired for analyte compound identification and quantitation, then measurement precision is improved, but measurement resolution and throughput deteriorate
Solution Approach 1:
The patent divides the comprehensive analysis into two separate measurement cycles: a first cycle for acquiring comprehensive data sets for identification, and a second cycle for rapid quantitation. This segmentation allows each cycle to be optimized for its specific purpose, resolving the contradiction between thorough identification and high-throughput quantitation
2Measurement precision
If comprehensive data sets are acquired for analyte compound identification and quantitation, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The patent segments the data acquisition process into two distinct measurement cycles with different acquisition parameters. The first cycle acquires comprehensive data for identification, while the second cycle acquires streamlined data for quantitation. This segmentation simplifies the overall system complexity by allowing each cycle to be independently optimized and controlled
Solution Approach 2:
The patent performs preliminary action by acquiring comprehensive identification data in the first measurement cycle before proceeding to the second cycle for quantitation. The results from the first cycle inform and guide the second cycle, allowing the system to leverage previously acquired information rather than repeating comprehensive measurements
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the quality of data by separating analyte quantitation and chemical assignment measurements, allowing for precise quantitation and chemical identification of analyte compounds, improving measurement resolution and throughput, and enabling the assignment of chemical names to analyte compounds with high confidence.
Implementation Method 1
subjecting an aliquot of pooled or otherwise composite sample to a first ion spectrometric measurement cycle, which disperses the analyte compounds according to a first number of physical-chemical properties (including mass analysis)
Data Source
AI summary
The disclosure relates generally to analytical measurement and, more specifically, to the use of ion spectrometry, including mass analysis, for the quantitation and chemical characterization (e.g., identification) of biological or synthetic materials using two different ion spectrometric measurement cycles. The methods may be employed fruitfully in particular with analyte molecules of low molecular weight, such as metabolites.