Ion Exchange Chromatography pH Excursion Control

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Solution Overview

Problem

Ion-exchange chromatography faces challenges with uncontrolled pH excursions, making it difficult to generate a collinear pH/conductivity gradient, which affects the quality of fractionation in cation and anion exchange chromatography.

Innovation Solution

Displacing hydrogen or hydroxide ions associated with the solid support using counterions of opposite charge before elution, thereby controlling pH and conductivity during the elution process, using buffers like Tris for cation exchange and acetate for anion exchange to maintain pH stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If sodium chloride is introduced into a cation exchange column, then conductivity rises linearly, but pH drops uncontrollably requiring many column volumes of buffer before normalization

Engineering Contradiction:
ImproveconductivityVSAvoidpH stability
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary action by pre-equilibrating the ion exchange resin with a buffer solution before introducing the salt gradient. This preliminary buffering step ensures that when sodium chloride is introduced, the pH remains stable because the resin is already saturated with buffer ions, preventing uncontrolled pH drops during elution.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses a buffer solution as an intermediary between the salt solution and the ion exchange resin. The buffer acts as a mediator that maintains pH stability while allowing the salt gradient to proceed, decoupling the conductivity increase from pH changes by introducing buffering capacity into the system.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If a salt gradient is applied to achieve elution, then conductivity increases linearly, but pH changes in an aberrant non-linear fashion reducing fractionation quality

Engineering Contradiction:
Improveelution efficiencyVSAvoidfractionation quality
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent changes the buffering parameters by using a buffer with sufficient capacity to maintain pH constant during the salt gradient elution. By adjusting the buffer concentration and pKa to match the elution conditions, the system achieves both efficient elution through conductivity increase and stable pH, enabling collinear pH/conductivity gradients for high-quality fractionation.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If buffer concentration is increased to maintain pH stability, then pH excursions are suppressed, but many column volumes of buffer are required increasing process time

Engineering Contradiction:
ImprovepH stabilityVSAvoidbuffer volume required
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent optimizes buffer parameters including concentration, pKa, and ionic strength to achieve effective pH control with minimal buffer volume. By selecting a buffer with appropriate pKa close to the operating pH and optimizing concentration, the system suppresses pH excursions efficiently without requiring excessive buffer volumes, reducing process time while maintaining pH stability.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method effectively suppresses pH excursions, allowing for controlled conductivity gradients without significant pH changes, improving the quality of fractionation and maintaining pH stability during elution.

Implementation Method 1

contacting the solid support with a solution comprising displacing counterions with a charge opposite the net charge of the solid support, wherein the displacing counter ion is a positively-charged molecule thereby replacing hydrogen ions associated with a negatively charged solid support, or wherein the displacing counter ion is a negatively-charged molecule thereby replacing hydroxide ions associated with a positively charged solid support

Methodology Applied
Scientific EffectIon Exchange: Ion Exchange

Implementation Method 2

subsequently eluting the target molecule from the solid support, wherein the eluting comprises increasing conductivity of an elution solution in contact with the solid support

Methodology Applied
Scientific EffectElectrical Conductivity: Conduction (electrical)

Data Source

PatentEP2814608B1REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY
Publication Date: 2018.11.14 BIO RAD LABORATORIES INC
  • EP2814608B1 patent drawingFigure 1
  • EP2814608B1 patent drawingFigure 2
  • EP2814608B1 patent drawingFigure 3

AI summary

Provided are methods and compositions for reducing or eliminating pH excursions in cation and anion exchange chromatography. Suppressing formation of uncontrolled pH excursions on a charged solid support having associated hydrogen or hydroxide ions is achieved by contacting the solid support with a solution comprising displacing counterions with a charge opposite the net charge of the solid support, thereby replacing hydrogen ions associated with a negatively charged solid support, or replacing hydroxide ions associated with a positively charged solid support. Subsequent eluting of a target molecule from the solid support by increasing conductivity of an elution solution does not lead to transient changes in effluent pH.